Rabbit Anti-Human Keratin 8 Polyclonal Antibody | MyBioSource MBS300531 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human Keratin 8 Polyclonal Antibody

catalog :

MBS300531

quantity :

7 ml

price :

220 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

product information

catalog number :

MBS300531

products type :

Antibody

products full name :

Rabbit Anti-Human Keratin 8 Polyclonal Antibody

products short name :

Keratin 8

other names :

keratin, type II cytoskeletal 8 isoform 1; Keratin, type II cytoskeletal 8; keratin, type II cytoskeletal 8; cytokeratin 8; cytokeratin-8; type-II keratin Kb8; keratin 8, type II; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8

other gene names :

KRT8; KRT8; K8; KO; CK8; CK-8; CYK8; K2C8; CARD2; CYK8; CK-8; K8

uniprot entry name :

K2C8_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human

sequence length :

511

form :

7.0 ml pre-diluted immunogen affinity purified rabbit polyclonal antibody in TBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide. (For IHConly)

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin, Western Blotting (WB)

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at RT. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: Ready To Use. IHC Positive Control: Lung, Skin, Colon, Breast Carcinoma. Western Blot . Recommended Starting Protocol: Dilute the antibody 1:25. Incubate for 1 hour at room temperature. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user. Flow Cytometry Positive Control: HeLa cell lysate

other info2 :

Immunogen: Recombinant protein encoding aa 1-483 of human cytokeratin 8. Control Tissue: Breast Carcinoma, Lung, Skin. Cellular Localization: Cytoplasm. Entrez Gene Code: 3856. Pathway: Stem Cell

products description :

Keratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with keratin 18. Keratin 8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize onlycytokeratin polypeptides 8 and 18.

ncbi gi num :

372466572

ncbi acc num :

NP_001243211.1

ncbi gb acc num :

NM_001256282.1

ncbi mol weight :

52 kDa

ncbi pathways :

EGFR1 Signaling Pathway 198782!!Signaling Mediated By P38-alpha And P38-beta Pathway 138012

ncbi summary :

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]

size :

7 ml

price :

220 USD