Rabbit Anti-Human mGluR5 Polyclonal Antibody | MyBioSource MBS300494 product information

product summary

request information :

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human mGluR5 Polyclonal Antibody

catalog :

MBS300494

quantity :

1 ml

price :

485 USD

clonality :

polyclonal

host :

rabbit

reactivity :

rat, dog, mouse, human

application :

immunohistochemistry - paraffin section, immunohistochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS300494

products type :

Antibody

products full name :

Rabbit Anti-Human mGluR5 Polyclonal Antibody

products short name :

mGluR5

other names :

metabotropic glutamate receptor 5 isoform b; Metabotropic glutamate receptor 5; metabotropic glutamate receptor 5; protein phosphatase 1, regulatory subunit 86; glutamate receptor, metabotropic 5; N/A

products gene name :

mGluR5

other gene names :

GRM5; GRM5; mGlu5; GPRC1E; MGLUR5; PPP1R86; GPRC1E; MGLUR5; mGluR5

uniprot entry name :

GRM5_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Bovine, Dog, Frog, Mouse, Rabbit, Rat

sequence length :

1180

form :

1.0 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:50. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue sections in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:50. IHC Positive Control: Brain

other info2 :

Immunogen: Synthetic peptide from C-terminus of human mGluR1. Control Tissue: Brain. Cellular Localization: Cytoplasm. Entrez Gene Code: 2915. Pathway: Neuroscience

products description :

Glutamate receptors constitute the principal excitatory neurotransmitter receptors in brain. Two classes of glutamate receptors exist: ionotropic receptors, and metabotropic receptors. Ionotropic glutamate receptors are oligomeric complexes of various subunits (GluR1-7, NMDA1-3, KAI-2) which comprise ligand-gated calcium channels. Metabotropic glutamate receptors are G-protein coupled receptors, which when activated can activate phopholipases or adenylylcyclase, depending on the neuron.

ncbi gi num :

4504143

ncbi acc num :

NP_000833.1

ncbi gb acc num :

NM_000842.4

ncbi mol weight :

110 kDa

ncbi pathways :

Calcium Signaling Pathway 83050!!Calcium Signaling Pathway 459!!Class C/3 (Metabotropic Glutamate/pheromone Receptors) Pathway 106381!!Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway 1127664!!Disease Pathway 530764!!G Alpha (q) Signalling Events Pathway 106043!!GPCR Downstream Signaling Pathway 119548!!GPCR Ligand Binding Pathway 161020!!GPCRs, Class C Metabotropic Glutamate, Pheromone Pathway 198839!!Gap Junction Pathway 83072

ncbi summary :

This gene encodes a member of the G-protein coupled receptor 3 protein family. The encoded protein is a metabatropic glutamate receptor, whose signaling activates a phosphatidylinositol-calcium second messenger system. This protein may be involved in the regulation of neural network activity and synaptic plasticity. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. A pseudogene of this gene has been defined on chromosome 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

size :

1 ml

price :

485 USD

more info or order :

MyBioSource product webpage