Rabbit Anti-Human PCNA Polyclonal Antibody | MyBioSource MBS300489 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human PCNA Polyclonal Antibody

catalog :

MBS300489

quantity :

7 ml

price :

220 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

product information

catalog number :

MBS300489

products type :

Antibody

products full name :

Rabbit Anti-Human PCNA Polyclonal Antibody

products short name :

PCNA

other names :

proliferating cell nuclear antigen; Proliferating cell nuclear antigen; proliferating cell nuclear antigen; DNA polymerase delta auxiliary protein; cyclin; proliferating cell nuclear antigen; Cyclin

products gene name :

PCNA

other gene names :

PCNA; PCNA; ATLD2; PCNA

uniprot entry name :

PCNA_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human

sequence length :

261

form :

7.0 ml pre-diluted immunogen affinity purified rabbit polyclonal antibody in TBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide. (For IHC only)

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin, Western Blotting (WB)

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: No special pretreatment necessary. Primary Antibody Incubation: Incubate for 10 minutes at RT. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: Ready To Use. IHC Positive Control: Tonsil, Lymph Node, Small Intestine. Western Blot . Recommended Starting Protocol: Dilute the antibody 1:25. Incubate for 1 hour at room temperature. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user. Western Blot Positive Control: 293T Cell Lysate

other info2 :

Immunogen: Recombinant human PCNA protein. Control Tissue: Small Intestine, Tonsil. Cellular Localization: Nucleus. Entrez Gene Code: 5111. Pathway: Cell Cycle

products description :

Expression of proliferating cell nuclear antigen (PCNA) or cyclin or polymerase delta auxiliary protein is elevated in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. PCNA/cyclin may act as an auxiliary protein of DNA polymerase-delta to play a fundamental role in the initiation of cell proliferation.

ncbi gi num :

4505641

ncbi acc num :

NP_002583.1

ncbi gb acc num :

NM_002592.2

ncbi mol weight :

30 kDa

ncbi pathways :

BARD1 Signaling Events Pathway 137959!!BRCA1-associated Genome Surveillance Complex (BASC) Pathway 413428!!BRCA1-associated Genome Surveillance Complex (BASC) Pathway 890555!!Base Excision Repair Pathway 105838!!Base Excision Repair Pathway 83043!!Base Excision Repair Pathway 451!!Cell Cycle Pathway 530733!!Cell Cycle, Mitotic Pathway 105765!!Cell Cycle Pathway 198811!!Cell Cycle Pathway 83054

ncbi summary :

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]

size :

7 ml

price :

220 USD