Rabbit Anti-Human Androgen Receptor Polyclonal Antibody | MyBioSource MBS300466 product information

product summary

request information :

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human Androgen Receptor Polyclonal Antibody

catalog :

MBS300466

quantity :

1 ml

price :

485 USD

clonality :

polyclonal

host :

rabbit

reactivity :

rat, mouse, human

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS300466

products type :

Antibody

products full name :

Rabbit Anti-Human Androgen Receptor Polyclonal Antibody

products short name :

Androgen Receptor

other names :

androgen receptor isoform 1; Androgen receptor; androgen receptor; androgen nuclear receptor variant 2; dihydrotestosterone receptor; nuclear receptor subfamily 3 group C member 4; androgen receptor; Dihydrotestosterone receptor; Nuclear receptor subfamily 3 group C member 4

products gene name :

other gene names :

AR; AR; KD; AIS; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA; DHTR; NR3C4

uniprot entry name :

ANDR_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Dog, Mouse, Pig, Rat

sequence length :

920

form :

1.0 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:200. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:200. IHC Positive Control: Prostate Carcinoma. Western Blot . Recommended Starting Protocol: Dilute the antibody 1:25. Incubate for 1 hour at room temperature. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user. Western Blot Positive Control: LnCap Cell Lysate

other info2 :

Immunogen: Synthetic peptide derived from near N-terminus of human AR. Control Tissue: Prostate Carcinoma. Cellular Localization: Nucleus. Entrez Gene Code: 367. Pathway: Hormones

products description :

The androgen receptor (AR) is a member of the steroid superfamily of ligand-dependent transcription factors. The expression of AR is reportedly inversely correlated with histologic grade. For example,.differentiated prostate tumors show higher expression than the poorly differentiated tumors.

ncbi gi num :

21322252

ncbi acc num :

NP_000035.2

ncbi gb acc num :

NM_000044.3

ncbi mol weight :

110 kDa

ncbi pathways :

Alpha6-Beta4 Integrin Signaling Pathway 198807!!Androgen Receptor Signaling Pathway 198806!!Coregulation Of Androgen Receptor Activity Pathway 138085!!FOXA1 Transcription Factor Network Pathway 137979!!Gene Expression Pathway 105937!!Generic Transcription Pathway 105938!!IL-6 Signaling Pathway 198864!!Integrated Breast Cancer Pathway 219801!!Integrated Pancreatic Cancer Pathway 711360!!Nongenotropic Androgen Signaling Pathway 138048

ncbi summary :

The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

size :

1 ml

price :

485 USD

more info or order :

MyBioSource product webpage