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antibody

Rabbit Anti-Human S100 Polyclonal Antibody

MBS300417

7 ml

220 USD

polyclonal

rabbit

immunohistochemistry, immunohistochemistry - paraffin section

MBS300417

Antibody

Rabbit Anti-Human S100 Polyclonal Antibody

S100

protein S100-A1; Protein S100-A1; protein S100-A1; S-100 protein alpha chain; S-100 protein subunit alpha; S100 alpha; S100 calcium-binding protein A1; S100 protein, alpha polypeptide; S100 calcium binding protein A1; S-100 protein alpha chain; S-100 protein subunit alpha; S100 calcium-binding protein A1

S100

S100A1; S100A1; S100; S100A; S100-alpha; S100A

S10A1_HUMAN

Polyclonal

IgG

Rabbit

Human

94

7.0 ml pre-diluted immunogen affinity purified rabbit polyclonal antibody in TBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

Immunohistochemistry (IHC) Paraffin

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:300. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: No special pretreatment necessary. Primary Antibody Incubation: Incubate for 10 minutes at RT. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: Ready To Use. IHC Positive Control: Melanoma, Schwannoma

Immunogen: Recombinant protein encoding human S100 protein. Control Tissue: Melanoma, Schwannoma. Cellular Localization: Cytoplasm. Entrez Gene Code: 6271. Pathway: Cancer Markers

S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. Antibody to S100 stains schwannomas, ependymomas, astrogliomas, almost all benign and malignant melanomas and their metastases. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes.

5454032

NP_006262.1

NM_006271.1

21-24 kDa

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies. [provided by RefSeq, Jul 2008]

7 ml

220 USD