This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MyBioSource
product type :
antibody
product name :
Mouse Anti-Human Actin-Skeletal Muscle Monoclonal Antibody (Clone 5C5.F8.C7)
catalog :
MBS300375
quantity :
1 ml
price :
410 USD
clonality :
monoclonal
host :
mouse
reactivity :
human
application :
immunohistochemistry, immunohistochemistry - paraffin section
product information
catalog number :
MBS300375
products type :
Antibody
products full name :
Mouse Anti-Human Actin-Skeletal Muscle Monoclonal Antibody (Clone 5C5.F8.C7)
products short name :
Actin-Skeletal Muscle
other names :
actin, alpha skeletal muscle; Actin, alpha skeletal muscle; actin, alpha skeletal muscle; nemaline myopathy type 3; actin, alpha 1, skeletal muscle; Alpha-actin-1
other gene names :
ACTA1; ACTA1; ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; CFTD1; CFTDM; ACTA
uniprot entry name :
ACTS_HUMAN
clonality :
Monoclonal
isotype :
IgM
clone :
5C5.F8.C7
host :
Mouse
reactivity :
Human
sequence length :
377
form :
1.0 ml mouse monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.
storage stability :
Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.
tested application :
Immunohistochemistry (IHC) Paraffin
app notes :
Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:50. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:50. IHC Positive Control: Skeletal Muscle
other info2 :
Immunogen: N-terminus decapeptide of alpha skeletal muscle isoform of actin. Control Tissue: Skeletal Muscle. Cellular Localization: Cytoplasm. Entrez Gene Code: 58. Pathway: Muscle Markers
products description :
This antibody reacts with sarcomeric actins of normal tissues and their tumors (i.e., rhabdomyosarcomas).
ncbi gi num :
4501881
ncbi acc num :
NP_001091.1
ncbi gb acc num :
NM_001100.3
ncbi mol weight :
42 kDa
ncbi pathways :
Caspase Cascade In Apoptosis Pathway 137974!!Hypothetical Network For Drug Addiction Pathway 198776!!Myometrial Relaxation And Contraction Pathways 198759!!RhoA Signaling Pathway 169356!!Signaling Events Mediated By Focal Adhesion Kinase Pathway 169348!!Striated Muscle Contraction Pathway 198903
ncbi summary :
The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008]
size :
1 ml
price :
410 USD
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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