Mouse Anti-Human Kappa Light Chain Monoclonal Antibody (Clone SPM508) | MyBioSource MBS300364 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Anti-Human Kappa Light Chain Monoclonal Antibody (Clone SPM508)

catalog :

MBS300364

quantity :

7 ml

price :

335 USD

clonality :

monoclonal

host :

mouse

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

product information

catalog number :

MBS300364

products type :

Antibody

products full name :

Mouse Anti-Human Kappa Light Chain Monoclonal Antibody (Clone SPM508)

products short name :

Kappa Light Chain

other names :

Ig kappa chain V-I region HK101; Ig kappa chain V-I region HK101; immunoglobulin kappa constant

other gene names :

IGKC; Km; HCAK1; IGKCD

uniprot entry name :

KV109_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

SPM508

host :

Mouse

reactivity :

Human

sequence length :

117

form :

7.0 ml pre-diluted mouse monoclonal antibody purified by protein A/G in TBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide. (For IHC only)

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:200. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: Ready To Use. IHC Positive Control: Tonsil. Western Blot . Recommended Starting Protocol: Dilute the antibody 1:25. Incubate for 1 hour at room temperature. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user. Western Blot Positive Control: Spleen Cell Lysate

other info2 :

Immunogen: B-lymphoma cells. Control Tissue: Tonsil. Cellular Localization: Cytoplasm. Entrez Gene Code: 3514. Pathway: Hematopoiesis

products description :

Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pentamer. IgA may also form polymers. Kappa light chain contains one immunoglobulin-like domain.

ncbi gi num :

125765

ncbi acc num :

P01601.1

ncbi mol weight :

25 kDa

ncbi pathways :

Adaptive Immune System Pathway 366160!!Antigen Activates B Cell Receptor (BCR) Leading To Generation Of Second Messengers Pathway 576249!!Binding And Uptake Of Ligands By Scavenger Receptors Pathway 771599!!Classical Antibody-mediated Complement Activation Pathway 106409!!Complement Cascade Pathway 106405!!Creation Of C4 And C2 Activators Pathway 106407!!FCERI Mediated Ca+2 Mobilization Pathway 833827!!FCERI Mediated MAPK Activation Pathway 833826!!FCERI Mediated NF-kB Activation Pathway 833828!!FCGR Activation Pathway 771578

size :

7 ml

price :

335 USD