product summary
request information :
company name :
MyBioSource
product type :
antibody
product name :
Rabbit Anti-Human Estrogen Receptor Polyclonal Antibody
catalog :
MBS300358
quantity :
1 ml
price :
410 USD
clonality :
polyclonal
host :
rabbit
reactivity :
human
application :
immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS300358
products type :
Antibody
products full name :
Rabbit Anti-Human Estrogen Receptor Polyclonal Antibody
products short name :
Estrogen Receptor
other names :
estrogen receptor isoform 1; Estrogen receptor; estrogen receptor; ER-alpha; estradiol receptor; estrogen nuclear receptor alpha; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; nuclear receptor subfamily 3 group A member 1; estrogen receptor 1; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1
products gene name :
ER
other gene names :
ESR1; ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1; ESR; NR3A1; ER
uniprot entry name :
ESR1_HUMAN
clonality :
Polyclonal
isotype :
IgG
host :
Rabbit
reactivity :
Human. Predicted Reactivity: Pig
sequence length :
595
form :
1.0 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.
storage stability :
Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.
tested application :
Immunohistochemistry (IHC) Paraffin
app notes :
Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at RT.Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Breast Carcinoma
other info2 :
Immunogen: Synthetic peptide derived from C-terminus of human estrogen receptor. Control Tissue: Breast Carcinoma. Cellular Localization: Nucleus. Entrez Gene Code: 2099. Pathway: Hormones
products description :
This antibody recognizes a protein of 67kDa, which is identified as estrogen receptor (ER). The ER gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains, labeled A through F. This antibody strongly stains the nucleus of epithelial cells in breast carcinomas. The ER is an important regulator of growth and differentiation in the mammary gland.
ncbi gi num :
62821794
ncbi acc num :
NP_000116.2
ncbi gb acc num :
NM_000125.3
ncbi mol weight :
67 kDa
ncbi pathways :
Endocrine And Other Factor-regulated Calcium Reabsorption Pathway 213307!!Endocrine And Other Factor-regulated Calcium Reabsorption Pathway 213276!!Estrogen Signaling Pathway 799177!!Estrogen Signaling Pathway 198863!!Estrogen Signaling Pathway 799197!!FOXA1 Transcription Factor Network Pathway 137979!!FOXM1 Transcription Factor Network Pathway 137935!!Gene Expression Pathway 105937!!Generic Transcription Pathway 105938!!Integrated Breast Cancer Pathway 219801
ncbi summary :
This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]
size :
1 ml
price :
410 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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