Rabbit Anti-Human ID2 Polyclonal Antibody | MyBioSource MBS300354 product information

product summary

request information :

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human ID2 Polyclonal Antibody

catalog :

MBS300354

quantity :

1 ml

price :

485 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, chicken, mouse, rat

application :

immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS300354

products type :

Antibody

products full name :

Rabbit Anti-Human ID2 Polyclonal Antibody

products short name :

ID2

other names :

DNA-binding protein inhibitor ID-2; DNA-binding protein inhibitor ID-2; DNA-binding protein inhibitor ID-2; DNA-binding protein inhibitor ID2; cell growth-inhibiting gene 8; class B basic helix-loop-helix protein 26; helix-loop-helix protein ID2; inhibitor of differentiation 2; inhibitor of DNA binding 2, dominant negative helix-loop-helix protein; Class B basic helix-loop-helix protein 26; bHLHb26; Inhibitor of DNA binding 2; Inhibitor of differentiation 2

products gene name :

ID2

other gene names :

ID2; ID2; GIG8; ID2A; ID2H; bHLHb26; BHLHB26; bHLHb26

uniprot entry name :

ID2_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Bovine, Chicken, Mouse, Pig, Rat

sequence length :

134

form :

1.0 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:50. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:50. IHC Positive Control: Breast Carcinoma

other info2 :

Immunogen: Synthetic peptide from C-terminus of human ID2 protein. Control Tissue: Breast Carcinoma. Cellular Localization: Nucleus. Entrez Gene Code: 3398. Pathway: Transcriptional Regulators

products description :

Inhibitor of differentiation (ID) proteins are transcriptional modulators that control differentiation and cell cycle progression in organisms ranging from flies to humans, and belong to a protein family characterized by a helix-loop-helix (HLH) region, but lacking the basic amino acid domain required to bind to DNA. ID2, but not its family members ID1 or ID3, can disrupt the anti-proliferative effects of retinoblastoma (Rb) family tumor suppressor proteins, thereby allowing progression of the cell cycle.

ncbi gi num :

31982933

ncbi acc num :

NP_002157.2

ncbi gb acc num :

NM_002166.4

ncbi mol weight :

15 kDa

ncbi pathways :

HIF-1-alpha Transcription Factor Network Pathway 138045!!Hippo Signaling Pathway 749777!!Hippo Signaling Pathway 750388!!Id Signaling Pathway 198871!!Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway 169352!!Signaling Pathways Regulating Pluripotency Of Stem Cells 1026136!!Signaling Pathways Regulating Pluripotency Of Stem Cells 1033502!!TGF-beta Signaling Pathway 83064!!TGF-beta Signaling Pathway 475!!Transcriptional Misregulation In Cancer Pathway 523016

ncbi summary :

The protein encoded by this gene belongs to the inhibitor of DNA binding family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the inhibitor of DNA binding family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene of this gene is located on chromosome 3. [provided by RefSeq, Aug 2011]

size :

1 ml

price :

485 USD

more info or order :

MyBioSource product webpage