catalog number :
MBS300188
products full name :
Rabbit Anti-Human Nrf2 Polyclonal Antibody
products short name :
NRF2
other names :
nuclear factor erythroid 2-related factor 2 isoform 2; Nuclear factor erythroid 2-related factor 2; nuclear factor erythroid 2-related factor 2; HEBP1; NF-E2-related factor 2; NFE2-related factor 2; nuclear factor (erythroid-derived 2)-like 2; nuclear factor erythroid-derived 2-like 2; nuclear factor, erythroid derived 2, like 2; nuclear factor, erythroid 2-like 2; HEBP1; Nuclear factor, erythroid derived 2, like 2
products gene name :
NRF2
other gene names :
NFE2L2; NFE2L2; NRF2; NRF2; NF-E2-related factor 2; NFE2-related factor 2
uniprot entry name :
NF2L2_HUMAN
reactivity :
Human. Predicted Reactivity: Bovine, Dog, Mouse, Pig, Rabbit, Rat
form :
1.0 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.
storage stability :
Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.
tested application :
Immunohistochemistry (IHC) Paraffin
app notes :
Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Placenta, Lung
other info2 :
Immunogen: Synthetic peptide corresponding to C-terminus of human NRF2 protein. Control Tissue: Placenta, Lung. Cellular Localization: Cytoplasm, Nucleus. Entrez Gene Code: 4780. Pathway: Transcriptional Regulators
products description :
Nfr1 (also designated TCF11), Nrf2 and Nfr3 are bZIP transcription factors that heterodimer with Maf proteins to bind MARE (Maf recognition elements) sequences. Nrf2 is widely expressed and is thought to translocate to the nucleus after treatment with xenobiotics and antioxidants, which stimulate its release from a repressor protein Keap1.
ncbi acc num :
NP_001138884.1
ncbi gb acc num :
NM_001145412.2
ncbi mol weight :
57/100 kDa
ncbi pathways :
AhR Pathway 755436!!Keap1-Nrf2 Pathway 198777!!Oxidative Stress Pathway 198916!!Protein Processing In Endoplasmic Reticulum Pathway 167325!!Protein Processing In Endoplasmic Reticulum Pathway 167190!!Selenium Metabolism And Selenoproteins Pathway 198846