Rabbit Anti-Human Calcitonin Polyclonal Antibody | MyBioSource MBS300151 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human Calcitonin Polyclonal Antibody

catalog :

MBS300151

quantity :

1 ml

price :

485 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

product information

catalog number :

MBS300151

products type :

Antibody

products full name :

Rabbit Anti-Human Calcitonin Polyclonal Antibody

products short name :

Calcitonin

other names :

calcitonin isoform CT preproprotein; Calcitonin; calcitonin; calcitonin gene-related peptide 1; alpha-type CGRP; calcitonin 1; calcitonin gene-related peptide I; calcitonin/calcitonin-related polypeptide, alpha; katacalcin; calcitonin-related polypeptide alpha; Calcitonin carboxyl-terminal peptide; CCP; PDN-21

other gene names :

CALCA; CALCA; CT; KC; CGRP; CALC1; CGRP1; CGRP-I; CALC1; CCP

uniprot entry name :

CALC_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Mouse, Rat

sequence length :

141

form :

1.0 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:300. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:300. IHC Positive Control: Thyroid, Medullary Carcinoma. Western Blot . Recommended Starting Protocol: Dilute the antibody 1:25. Incubate for 1 hour at room temperature. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user. Western Blot Positive Control: Thyroid Cell Lysate

other info2 :

Immunogen: Synthetic human calcitonin 1-32 amino acid peptide. Control Tissue: Thyroid, Thyroid Medullary Carcinoma. Cellular Localization: Cytoplasm. Entrez Gene Code: 796. Pathway: Hormones

products description :

Calcitonin is a 32 amino acid peptide which can be demonstrated in C cells of the normal and hyperplastic thyroid. Staining for calcitonin may be used for the identification of a spectrum of C cell proliferative abnormalities ranging from C cell hyperplasia to invasive tumors. Staining for calcitonin in medullary carcinoma of the thyroid produces a fine granular pattern in the cytoplasm. Amyloid deposits within the tumor may also exhibit varying degrees of calcitonin activity.

ncbi gi num :

76880484

ncbi acc num :

NP_001029124.1

ncbi gb acc num :

NM_001033952.2

ncbi mol weight :

14 kDa

ncbi pathways :

Amyloids Pathway 366238!!Calcitonin-like Ligand Receptors Pathway 106379!!Class B/2 (Secretin Family Receptors) Pathway 106378!!Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway 1127664!!Disease Pathway 530764!!Endothelin Pathway 672453!!G Alpha (s) Signalling Events Pathway 119549!!GPCR Downstream Signaling Pathway 119548!!GPCR Ligand Binding Pathway 161020!!Metabolic Disorders Of Biological Oxidation Enzymes Pathway 1127637

ncbi summary :

This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator and as an antimicrobial peptide while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2014]

size :

1 ml

price :

485 USD