Human Hyaluronan synthase 1 ELISA Kit | MyBioSource MBS289944 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Hyaluronan synthase 1 ELISA Kit

catalog :

MBS289944

quantity :

48-Strip-Wells

price :

400 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS289944

products type :

ELISA Kit

products full name :

Human Hyaluronan synthase 1 ELISA Kit

products short name :

[Hyaluronan synthase 1]

products name syn :

[Hyaluronan synthase 1; 2.4.1.212; Hyaluronate synthase 1; Hyaluronic acid synthase 1; HA synthase 1; HAS1; HAS]

other names :

[hyaluronan synthase 1 isoform 1; Hyaluronan synthase 1; hyaluronan synthase 1; hyaluronan synthase 1; Hyaluronate synthase 1; Hyaluronic acid synthase 1; HA synthase 1; HuHAS1]

products gene name :

[HAS1]

other gene names :

[HAS1; HAS1; HAS; HAS; HA synthase 1; HuHAS1]

uniprot entry name :

HYAS1_HUMAN

reactivity :

Human

sequence length :

578

storage stability :

For long term storage, please store the entire kit at -20 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Assay Type: Sandwich. Detection Range: 0.156-10 ng/mL. Sensitivity: 0.098ng/mL

other info2 :

Intra-Assay CV: <=4.9%. Inter-Assay CV: <=9.5%. Recovery: 107%

products description :

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

167466171

ncbi acc num :

NP_001514.2

ncbi gb acc num :

NM_001523.3

uniprot acc num :

Q92839

ncbi mol weight :

64,832 Da

ncbi pathways :

Glycosaminoglycan Metabolism Pathway (1269972); Hyaluronan Biosynthesis And Export Pathway (1269974); Hyaluronan Metabolism Pathway (1269973); Metabolism Pathway (1269956); Metabolism Of Carbohydrates Pathway (1269957)

ncbi summary :

Hyaluronan or hyaluronic acid (HA) is a high molecular weight unbranched polysaccharide synthesized by a wide variety of organisms from bacteria to mammals, and is a constituent of the extracellular matrix. It consists of alternating glucuronic acid and N-acetylglucosamine residues that are linked by beta-1-3 and beta-1-4 glycosidic bonds. HA is synthesized by membrane-bound synthase at the inner surface of the plasma membrane, and the chains are extruded through pore-like structures into the extracellular space. It serves a variety of functions, including space filling, lubrication of joints, and provision of a matrix through which cells can migrate. HA is actively produced during wound healing and tissue repair to provide a framework for ingrowth of blood vessels and fibroblasts. Changes in the serum concentration of HA are associated with inflammatory and degenerative arthropathies such as rheumatoid arthritis. In addition, the interaction of HA with the leukocyte receptor CD44 is important in tissue-specific homing by leukocytes, and overexpression of HA receptors has been correlated with tumor metastasis. HAS1 is a member of the newly identified vertebrate gene family encoding putative hyaluronan synthases, and its amino acid sequence shows significant homology to the hasA gene product of Streptococcus pyogenes, a glycosaminoglycan synthetase (DG42) from Xenopus laevis, and a recently described murine hyaluronan synthase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

uniprot summary :

HAS1: Plays a role in hyaluronan/hyaluronic acid (HA) synthesis. Also able to catalyze the synthesis of chito- oligosaccharide depending on the substrate. Belongs to the NodC/HAS family. Protein type: Cell adhesion; EC 2.4.1.212; Motility/polarity/chemotaxis; Membrane protein, integral; Membrane protein, multi-pass; Transferase. Chromosomal Location of Human Ortholog: 19q13.4. Cellular Component: cytoplasm; integral to plasma membrane; plasma membrane. Molecular Function: hyaluronan synthase activity. Biological Process: carbohydrate metabolic process; cell adhesion; extracellular polysaccharide biosynthetic process; glycosaminoglycan biosynthetic process; glycosaminoglycan metabolic process; hyaluronan biosynthetic process; hyaluronan metabolic process

size1 :

48-Strip-Wells

price1 :

400 USD

size2 :

96-Strip-Wells

price2 :

530

size3 :

5x96-Strip-Wells

price3 :

2305

size4 :

10x96-Strip-Wells

price4 :

4250

more info or order :

MyBioSource product webpage