catalog number :
MBS289817
products type :
ELISA Kit
products full name :
Rat Arginase-1 ELISA Kit
products short name :
[Arginase-1]
products name syn :
[Arginase-1; Liver-type arginase; Type I arginase; Arg1; 3.5.3.1]
other names :
[arginase-1; Arginase-1; arginase-1; arginase 1; Liver-type arginase; Type I arginase]
products gene name :
[Arg1]
other gene names :
[Arg1; Arg1]
uniprot entry name :
ARGI1_RAT
specificity :
Recombinant and natural Rat Arginase-1
storage stability :
For long term storage, please store the entire kit at -20 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids.
products description :
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_058830.2
ncbi gb acc num :
NM_017134.3
ncbi mol weight :
34,973 Da
ncbi pathways :
Amoebiasis Pathway (167311); Amoebiasis Pathway (167191); Arginine And Proline Metabolism Pathway (83349); Arginine And Proline Metabolism Pathway (323); Arginine Biosynthesis Pathway (83336); Arginine Biosynthesis Pathway (306); Biosynthesis Of Amino Acids Pathway (791250); Biosynthesis Of Amino Acids Pathway (795174); Metabolic Pathways (132933); Metabolism Pathway (1333271)
ncbi summary :
catalyzes the hydrolysis of arginine to ornithine and urea in arginine metabolism; regulates nitric oxide production [RGD, Feb 2006]
uniprot summary :
ARG1: Homotrimer. By arginine or homoarginine. Belongs to the arginase family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Amino Acid Metabolism - arginine and proline; EC 3.5.3.1; Hydrolase. Cellular Component: cell soma; cytoplasm; extracellular space; mitochondrial outer membrane; neuron projection; nucleus. Molecular Function: arginase activity; manganese ion binding. Biological Process: aging; arginine catabolic process to ornithine; arginine metabolic process; collagen biosynthetic process; female pregnancy; liver development; lung development; mammary gland involution; maternal process involved in pregnancy; positive regulation of endothelial cell proliferation; response to amine stimulus; response to amino acid stimulus; response to axon injury; response to cadmium ion; response to drug; response to herbicide; response to lipopolysaccharide; response to manganese ion; response to methylmercury; response to peptide hormone stimulus; response to selenium ion; response to steroid hormone stimulus; response to vitamin A; response to vitamin E; response to wounding; response to zinc ion; urea cycle
size4 :
10x96-Strip-Wells