ELISA/assay

Mouse Tripartite motif-containing protein 72 ELISA Kit

MBS289666

48-Strip-Wells

355 USD

ELISA Kit

Mouse Tripartite motif-containing protein 72 ELISA Kit

[Tripartite motif-containing protein 72]

[Tripartite motif-containing protein 72; Mitsugumin-53; Mg53; Trim72; Mg53]

[tripartite motif-containing protein 72; Tripartite motif-containing protein 72; tripartite motif-containing protein 72; tripartite motif-containing 72; Mitsugumin-53; Mg53]

[Trim72]

[Trim72; Trim72; MG53; BC067209; Mg53]

TRI72_MOUSE

Mouse

477

For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids.

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

121247302

NP_001073401.1

NM_001079932.3

Q1XH17

52,817 Da

Muscle Contraction Pathway (1323437); Smooth Muscle Contraction Pathway (1323439)

TRIM72: Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles. Belongs to the TRIM/RBCC family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Ubiquitin ligase; Ubiquitin conjugating system. Cellular Component: cytoplasmic vesicle; cytoplasmic vesicle membrane; intracellular; membrane; plasma membrane; sarcolemma. Molecular Function: metal ion binding; phosphatidylserine binding; protein binding; ubiquitin conjugating enzyme binding; zinc ion binding. Biological Process: exocytosis; membrane budding; muscle development; muscle system process; negative regulation of insulin receptor signaling pathway; negative regulation of insulin-like growth factor receptor signaling pathway; plasma membrane repair; proteasomal ubiquitin-dependent protein catabolic process; protein homooligomerization; protein ubiquitination; transport

48-Strip-Wells

355 USD

96-Strip-Wells

475

5x96-Strip-Wells

2030

10x96-Strip-Wells

3700