ELISA/assay

Rat Superoxide dismutase [Cu-Zn] ELISA Kit

MBS289512

48-Strip-Wells

355 USD

ELISA Kit

Rat Superoxide dismutase [Cu-Zn] ELISA Kit

[Superoxide dismutase [Cu-Zn]]

[Superoxide dismutase [Cu-Zn]; Sod1; 1.15.1.1]

[superoxide dismutase; Superoxide dismutase [Cu-Zn]; superoxide dismutase [Cu-Zn]; superoxide dismutase 1, soluble]

[Sod1]

[Sod1; Sod1; CuZnSOD]

SODC_RAT

Rat

154

For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Assay Type: Competitive. Detection Range: 1.56-100 U/mL. Sensitivity: 0.78 U/mL

Intra-Assay CV: <=5.6%. Inter-Assay CV: <=9.6%. Recovery: 89%

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen, During the reaction, target antigen in the sample or standard competes with a fixed amount of biotin-labeled target antigen for sites on a pre-coated antibody specific to target antigen. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

8394328

NP_058746.1

NM_017050.1

P07632

15,912 Da

Amyotrophic Lateral Sclerosis (ALS) Pathway (83491); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Cellular Responses To Stress Pathway (1333252); Detoxification Of Reactive Oxygen Species Pathway (1333258); Folic Acid Network Pathway (219799); Hemostasis Pathway (1333034); Huntington's Disease Pathway (83492); Huntington's Disease Pathway (512); Longevity Regulating Pathway - Multiple Species (1340755); Longevity Regulating Pathway - Multiple Species (1340651)

catalyzes the conversion of superoxide to hydrogen peroxide and molecular oxygen; involved in response to oxidative stress [RGD, Feb 2006]

SOD1: Destroys radicals which are normally produced within the cells and which are toxic to biological systems. Homodimer; non-disulfide linked. Homodimerization may take place via the ditryptophan cross-link at Trp-33. The pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 interact with RNF19A, whereas wild-type protein does not. The pathogenic variants ALS1 Arg-86 and Ala-94 interact with MARCH5, whereas wild-type protein does not. Belongs to the Cu-Zn superoxide dismutase family. Protein type: Oxidoreductase; EC 1.15.1.1; Nuclear receptor co-regulator; Apoptosis; Mitochondrial. Cellular Component: cell soma; cytoplasm; cytoplasmic vesicle; cytosol; dendrite cytoplasm; extracellular matrix; extracellular region; extracellular space; lysosome; mitochondrial intermembrane space; mitochondrion; myelin sheath; neuron projection; nucleoplasm; nucleus; peroxisome; plasma membrane; protein complex; secretory granule. Molecular Function: chaperone binding; copper ion binding; enzyme binding; identical protein binding; protein phosphatase 2B binding; Rac GTPase binding; superoxide dismutase activity; zinc ion binding. Biological Process: activation of MAPK activity; aging; anterograde axon cargo transport; auditory receptor cell stereocilium organization and biogenesis; cell aging; cellular iron ion homeostasis; DNA fragmentation during apoptosis; double-strand break repair; embryo implantation; glutathione metabolic process; heart contraction; hydrogen peroxide biosynthetic process; locomotory behavior; muscle maintenance; myelin maintenance in the peripheral nervous system; myeloid cell homeostasis; negative regulation of apoptosis; negative regulation of cholesterol biosynthetic process; negative regulation of neuron apoptosis; neurofilament cytoskeleton organization and biogenesis; ovarian follicle development; positive regulation of catalytic activity; positive regulation of cytokine production; positive regulation of superoxide release; regulation of blood pressure; regulation of GTPase activity; regulation of mitochondrial membrane potential; regulation of multicellular organism growth; regulation of protein kinase activity; relaxation of vascular smooth muscle; removal of superoxide radicals; response to amphetamine; response to antibiotic; response to axon injury; response to copper ion; response to drug; response to ethanol; response to heat; response to hydrogen peroxide; response to nutrient levels; response to organic substance; response to oxidative stress; response to reactive oxygen species; response to superoxide; retinal homeostasis; retrograde axon cargo transport; sensory perception of sound; spermatogenesis; superoxide metabolic process; superoxide release; transmission of nerve impulse

48-Strip-Wells

355 USD

96-Strip-Wells

475

5x96-Strip-Wells

2030

10x96-Strip-Wells

3700