ELISA/assay

Pig Insulin-like growth factor I ELISA Kit

MBS289333

48-Strip-Wells

355 USD

ELISA Kit

Pig Insulin-like growth factor I ELISA Kit

[Insulin-like growth factor I]

[Insulin-like growth factor I; IGF-I; Somatomedin; IGF1]

[insulin-like growth factor I; Insulin-like growth factor I; insulin-like growth factor I; Somatomedin]

[IGF1]

[IGF1; IGF1; IGF-1; IGF-I; Npt2B; IGF-I]

IGF1_PIG

Pig

130

For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids.

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

47523588

NP_999421.1

NM_214256.1

P16545

17,010 Da

AMPK Signaling Pathway (989938); AMPK Signaling Pathway (992181); Aldosterone-regulated Sodium Reabsorption Pathway (130624); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Dilated Cardiomyopathy Pathway (121477); Dilated Cardiomyopathy Pathway (121285); Focal Adhesion Pathway (84479); Focal Adhesion Pathway (478); FoxO Signaling Pathway (921549); Glioma Pathway (84517)

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca2+-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 ().

48-Strip-Wells

355 USD

96-Strip-Wells

475

5x96-Strip-Wells

2030

10x96-Strip-Wells

3700