Human HLA class I histocompatibility antigen, alpha chain G ELISA Kit | MyBioSource MBS289314 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human HLA class I histocompatibility antigen, alpha chain G ELISA Kit

catalog :

MBS289314

quantity :

48-Strip-Wells

price :

355 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS289314

products type :

ELISA Kit

products full name :

Human HLA class I histocompatibility antigen, alpha chain G ELISA Kit

products short name :

[HLA class I histocompatibility antigen, alpha chain G]

products name syn :

[HLA class I histocompatibility antigen, alpha chain G; HLA G antigen; MHC class I antigen G; HLA-G; HLA-6.0; HLAG]

other names :

[HLA class I histocompatibility antigen, alpha chain G; HLA class I histocompatibility antigen, alpha chain G; HLA class I histocompatibility antigen, alpha chain G; major histocompatibility complex, class I, G; HLA G antigen; MHC class I antigen G]

products gene name :

[HLA-G]

other gene names :

[HLA-G; HLA-G; MHC-G; HLA-6.0; HLAG]

uniprot entry name :

HLAG_HUMAN

reactivity :

Human

sequence length :

338

storage stability :

For long term storage, please store the entire kit at -20 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Assay Type: Sandwich. Detection Range: 0.312-20 ng/mL. Sensitivity: 0.11ng/mL

other info2 :

Intra-Assay CV: <=5.6%. Inter-Assay CV: <=8.9%. Recovery: 97%

products description :

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human HLA-G concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids. Principle of the Assay The microtiter plate provided in this kit has been pre-coated with an antibody specific to HLA-G. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for HLA-G and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HLA-G, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of HLA-G in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

4504415

ncbi acc num :

NP_002118.1

ncbi gb acc num :

NM_002127.5

uniprot acc num :

P17693

ncbi mol weight :

38,224 Da

ncbi pathways :

Adaptive Immune System Pathway (1269171); Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway (1269194); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Antigen Processing-Cross Presentation Pathway (1269195); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533)

ncbi summary :

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]

uniprot summary :

HLA-G: Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T-lymphocytes, macrophages and mononuclear cells. Belongs to the MHC class I family. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: early endosome membrane; Golgi membrane; membrane; MHC class I protein complex; phagocytic vesicle membrane; plasma membrane. Molecular Function: antigen binding; peptide antigen binding; protein homodimerization activity; receptor binding. Biological Process: antigen processing and presentation; antigen processing and presentation of exogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of peptide antigen via MHC class I; cellular defense response; cytokine and chemokine mediated signaling pathway; immune response-inhibiting cell surface receptor signaling pathway; negative regulation of immune response; negative regulation of T cell proliferation; positive regulation of interleukin-12 production; positive regulation of regulatory T cell differentiation; positive regulation of T cell tolerance induction; positive regulation of tolerance induction; regulation of immune response. Disease: Asthma, Susceptibility To

size1 :

48-Strip-Wells

price1 :

355 USD

size2 :

96-Strip-Wells

price2 :

475

size3 :

5x96-Strip-Wells

price3 :

2030

size4 :

10x96-Strip-Wells

price4 :

3700

more info or order :

MyBioSource product webpage