ELISA/assay

Mouse Pigment epithelium-derived factor ELISA Kit

MBS2887741

48-Strip-Wells

355 USD

ELISA Kit

Mouse Pigment epithelium-derived factor ELISA Kit

[Pigment epithelium-derived factor]

[Pigment epithelium-derived factor; PEDF; Caspin; Serpin F1; Stromal cell-derived factor 3; SDF-3; Serpinf1; Pedf; Sdf3]

[pigment epithelium-derived factor; Pigment epithelium-derived factor; Caspin; Serpin F1; Stromal cell-derived factor 3; SDF-3]

[Serpinf1]

[Serpinf1; Pedf; Sdf3; PEDF; SDF-3]

PEDF_MOUSE

Mouse

417

Recombinant and natural Human VEGF-B

For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids. Detection Range: 15.6-1000 pg/mL. Sensitivity: <6.1 pg/mL

Intra-assay Precision: Intra-Assay CV: <=5.3%. Inter-assay Precision: Inter-Assay CV: <=9.5%

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

117606335

NP_035470.3

NM_011340.3

P97298

46,234 Da

Wnt Signaling Pathway (83258); Wnt Signaling Pathway (471)

PEDF: a secreted neurotrophic protein that induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity. Extracellular phosphorylation enhances antiangiogenic activity. Protein type: Inhibitor; Secreted; Secreted, signal peptide. Cellular Component: axon; axon hillock; basement membrane; cell soma; extracellular matrix; extracellular region; extracellular space; perinuclear region of cytoplasm. Molecular Function: serine-type endopeptidase inhibitor activity. Biological Process: negative regulation of angiogenesis; negative regulation of inflammatory response; positive regulation of neurogenesis; short-term memory

48-Strip-Wells

355 USD

96-Strip-Wells

475

5x96-Strip-Wells

2030

10x96-Strip-Wells

3700