catalog number :
MBS2887588
products type :
ELISA Kit
products full name :
Mouse Connective tissue growth factor ELISA Kit
products short name :
[Connective tissue growth factor]
products name syn :
[Connective tissue growth factor; CCN family member 2; Hypertrophic chondrocyte-specific protein 24; Protein FISP-12; Ctgf; Ccn2; Fisp-12; Fisp12; Hcs24]
other names :
[connective tissue growth factor; Connective tissue growth factor; connective tissue growth factor; connective tissue growth factor; CCN family member 2; Hypertrophic chondrocyte-specific protein 24; Protein FISP-12]
products gene name :
[Ctgf]
other gene names :
[Ctgf; Ctgf; Ccn2; Hcs24; Fisp12; fisp-12; Ccn2; Fisp-12; Fisp12; Hcs24]
uniprot entry name :
CTGF_MOUSE
specificity :
Recombinant and natural Mouse Connective tissue growth factor
storage stability :
For long term storage, please store the entire kit at -20 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Detection Range: 15.6-1000 pg/mL. Sensitivity: <7.8 pg/mL
other info2 :
Intra-assay Precision: Intra-Assay CV: <5.8%. Inter-assay Precision: Inter-Assay CV: <9.2%
products description :
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_034347.2
ncbi gb acc num :
NM_010217.2
ncbi mol weight :
37,824 Da
ncbi pathways :
Hippo Signaling Pathway (749786); Hippo Signaling Pathway (750388); PodNet: Protein-protein Interactions In The Podocyte Pathway (755428); XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway (755427)
uniprot summary :
CTGF: Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis. Belongs to the CCN family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cell adhesion; Secreted; Secreted, signal peptide. Cellular Component: cell cortex; cis-Golgi network; cytosol; extracellular region; extracellular space; Golgi apparatus; intracellular membrane-bound organelle; perinuclear region of cytoplasm; proteinaceous extracellular matrix. Molecular Function: fibronectin binding; growth factor activity; heparin binding; insulin-like growth factor binding; integrin binding; protein C-terminus binding. Biological Process: angiogenesis; cartilage condensation; cell adhesion; cell differentiation; cell migration; cell-cell signaling; cell-matrix adhesion; fibroblast growth factor receptor signaling pathway; integrin-mediated signaling pathway; lung development; ossification; positive regulation of caspase activity; positive regulation of cell activation; positive regulation of cell differentiation; positive regulation of cell proliferation; positive regulation of collagen biosynthetic process; positive regulation of JNK cascade; positive regulation of protein amino acid phosphorylation; positive regulation of stress fiber formation; regulation of cell growth; regulation of chondrocyte differentiation; tissue homeostasis
size4 :
10x96-Strip-Wells