ELISA/assay

Mouse Transcription factor 7-like 2 ELISA Kit

MBS2887486

48-Strip-Wells

400 USD

ELISA Kit

Mouse Transcription factor 7-like 2 ELISA Kit

[Transcription factor 7-like 2]

[Transcription factor 7-like 2; HMG box transcription factor 4; T-cell-specific transcription factor 4; T-cell factor 4; TCF-4; mTCF-4; Tcf7l2; Tcf4]

[transcription factor 7-like 2 isoform 1; Transcription factor 7-like 2; transcription factor 7-like 2; transcription factor 7 like 2, T cell specific, HMG box; HMG box transcription factor 4; T-cell-specific transcription factor 4; T-cell factor 4; TCF-4; mTCF-4]

[Tcf7l2]

[Tcf7l2; Tcf7l2; Tcf4; TCF4B; TCF4E; Tcf-4; mTcf-4B; mTcf-4E; Tcf4; T-cell factor 4; TCF-4; mTCF-4]

TF7L2_MOUSE

Mouse

459

For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Assay Type: Sandwich. Detection Range: 62.5-4000 pg/mL. Sensitivity: 31.25 pg/mL

Intra-Assay CV: <=6.1%. Inter-Assay CV: <=8.8%. Recovery: 100%

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

218931182

NP_001136390.1

NM_001142918.1

Q924A0

33,726 Da

Acute Myeloid Leukemia Pathway (83310); Acute Myeloid Leukemia Pathway (529); Adherens Junction Pathway (83267); Adherens Junction Pathway (481); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (117303); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (116129); Basal Cell Carcinoma Pathway (83306); Basal Cell Carcinoma Pathway (525); Beta-catenin Independent WNT Signaling Pathway (1324769); Ca2+ Pathway (1324774)

TCF7L2: Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine. Interacts with TGFB1I1. Interacts with CTNNB1 (via the armadillo repeat); forms stable transcription complex. Interacts with EP300. Interacts with NLK. Interacts with CCDC85B (probably through the HMG box); prevents interaction with CTNNB1. Interacts with TNIK. Interacts with MAD2L2; prevents TCF7L2/TCF4 binding to promZIPK/DAPK3oters, negatively modulating its transcriptional activity. Interacts with ZIPK/DAPK3. Interacts with XIAP/BIRC4 and TLE3. Detected in epithelium from small intestine, with the highest expression at the top of the crypts and a gradient of expression from crypt to villus. Detected in colon epithelium and colon cancer, and in epithelium from mammary gland and carcinomas derived therefrom. Belongs to the TCF/LEF family. 10 isoforms of the human protein are produced by alternative splicing. Protein type: Transcription factor; Motility/polarity/chemotaxis; DNA-binding; Cell development/differentiation; Cell cycle regulation; Apoptosis. Cellular Component: cytoplasm; cytosol; nuclear chromatin; nucleoplasm; nucleus; transcription factor complex. Molecular Function: beta-catenin binding; chromatin binding; DNA binding; gamma-catenin binding; nuclear hormone receptor binding; protein binding; protein kinase binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding. Biological Process: blood vessel development; bone mineralization; cell cycle arrest; cell glucose homeostasis; cell proliferation; cellular response to starvation; embryonic digestive tract morphogenesis; embryonic genitalia morphogenesis; embryonic hindgut morphogenesis; fat cell differentiation; glucose homeostasis; glucose metabolic process; glycogen metabolic process; maintenance of DNA repeat elements; multicellular organism growth; myoblast cell fate commitment; negative regulation of BMP signaling pathway; negative regulation of fat cell differentiation; negative regulation of fibroblast growth factor receptor signaling pathway; negative regulation of organ growth; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; neural tube development; odontogenesis of dentine-containing teeth; oligodendrocyte development; pituitary gland development; positive regulation of apoptosis; positive regulation of epithelial cell proliferation; positive regulation of gluconeogenesis; positive regulation of insulin secretion; positive regulation of lipid biosynthetic process; positive regulation of protein binding; positive regulation of protein export from nucleus; positive regulation of protein kinase B signaling cascade; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; post-embryonic development; regulation of hormone metabolic process; regulation of myelination; regulation of oligodendrocyte differentiation; regulation of skeletal muscle development; regulation of smooth muscle cell proliferation; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; response to glucose stimulus; secretory granule localization; skin development; somatic stem cell maintenance; transcription, DNA-dependent; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin

48-Strip-Wells

400 USD

96-Strip-Wells

530

5x96-Strip-Wells

2305

10x96-Strip-Wells

4250