catalog number :
MBS2887434
products type :
ELISA Kit
products full name :
Bovine Vascular endothelial growth factor A ELISA Kit
products short name :
[Vascular endothelial growth factor A]
products name syn :
[Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF; VEGFA; VEGF]
other names :
[vascular endothelial growth factor A isoform VEGF-A; Vascular endothelial growth factor A; vascular endothelial growth factor A; Vascular permeability factor; VPF]
products gene name :
[VEGFA]
other gene names :
[VEGFA; VEGFA; VPF; VEGF; VEGF-A; eVEGF120; eVEGF164; VEGF; VEGF-A; VPF]
uniprot entry name :
VEGFA_BOVIN
storage stability :
For long term storage, please store the entire kit at -20 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids.
products description :
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_776641.1
ncbi gb acc num :
NM_174216.2
ncbi mol weight :
17,086 Da
ncbi pathways :
AGE-RAGE Signaling Pathway In Diabetic Complications (1319849); AGE-RAGE Signaling Pathway In Diabetic Complications (1319775); Bladder Cancer Pathway (84350); Bladder Cancer Pathway (527); Cytokine-cytokine Receptor Interaction Pathway (84290); Cytokine-cytokine Receptor Interaction Pathway (460); Endochondral Ossification Pathway (920938); Focal Adhesion Pathway (84306); Focal Adhesion Pathway (478); HIF-1 Signaling Pathway (695207)
ncbi summary :
This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. It is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. [provided by RefSeq, Nov 2015]
uniprot summary :
Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin ().
size4 :
10x96-Strip-Wells