ELISA/assay

Rat Troponin T, cardiac muscle ELISA Kit

MBS2887270

48-Strip-Wells

355 USD

ELISA Kit

Rat Troponin T, cardiac muscle ELISA Kit

[Troponin T, cardiac muscle]

[Troponin T, cardiac muscle; TnTc; Cardiac muscle troponin T; cTnT; Tnnt2]

[troponin T, cardiac muscle; Troponin T, cardiac muscle; troponin T, cardiac muscle; troponin T2, cardiac type; Cardiac muscle troponin T; cTnT]

[Tnnt2]

[Tnnt2; Tnnt2; Ctt; CTTG; Tnnt3; RATCTTG; TnTc; cTnT]

TNNT2_RAT

Rat

299

For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Assay Type: Sandwich. Detection Range: 1.56-100 pg/mL. Sensitivity: 0.781 pg/mL

Intra-Assay CV: <=4.2%. Inter-Assay CV: <=7.8%. Recovery: 89%

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

6981666

NP_036808.1

NM_012676.1

P50753

34,452 Da

Adrenergic Signaling In Cardiomyocytes Pathway (908278); Adrenergic Signaling In Cardiomyocytes Pathway (909696); Cardiac Muscle Contraction Pathway (93346); Cardiac Muscle Contraction Pathway (93992); Dilated Cardiomyopathy Pathway (121476); Dilated Cardiomyopathy Pathway (121285); Hypertrophic Cardiomyopathy (HCM) Pathway (114014); Hypertrophic Cardiomyopathy (HCM) Pathway (106591); Muscle Contraction Pathway (1333549); Striated Muscle Contraction Pathway (1333550)

tropomyosin-binding subunit of troponin; confers calcium-sensitivy to actinomysin ATPase activity in striated muscle [RGD, Feb 2006]

TNNT2: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart. Belongs to the troponin T family. 11 isoforms of the human protein are produced by alternative splicing. Protein type: Motor; Motility/polarity/chemotaxis. Cellular Component: cytoplasm; myofibril; sarcomere; striated muscle thin filament; troponin complex. Molecular Function: actin binding; ATPase activity; protein binding, bridging; structural constituent of cytoskeleton; tropomyosin binding; troponin C binding; troponin I binding. Biological Process: actin crosslink formation; atrial cardiac muscle morphogenesis; cardiac muscle contraction; heart development; muscle contraction; muscle filament sliding; negative regulation of ATPase activity; positive regulation of ATPase activity; protein heterooligomerization; regulation of heart contraction; regulation of muscle contraction; regulation of muscle filament sliding speed; response to calcium ion; sarcomere organization; ventricular cardiac muscle morphogenesis

48-Strip-Wells

355 USD

96-Strip-Wells

475

5x96-Strip-Wells

2030

10x96-Strip-Wells

3700