Human Cofilin-1 ELISA Kit | MyBioSource MBS2886911 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Cofilin-1 ELISA Kit

catalog :

MBS2886911

quantity :

48-Strip-Wells

price :

355 USD

product information

catalog number :

MBS2886911

products type :

ELISA Kit

products full name :

Human Cofilin-1 ELISA Kit

products short name :

[Cofilin-1]

products name syn :

[Cofilin-1; 18 kDa phosphoprotein; p18; Cofilin, non-muscle isoform; CFL1; CFL]

other names :

[cofilin-1; Cofilin-1; cofilin 1; 18 kDa phosphoprotein; p18; Cofilin, non-muscle isoform]

products gene name :

[CFL1]

other gene names :

[CFL1; CFL1; CFL; cofilin; HEL-S-15; CFL; p18]

uniprot entry name :

COF1_HUMAN

reactivity :

Human

sequence length :

166

storage stability :

For long term storage, please store the entire kit at -20 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids.

products description :

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

5031635

ncbi acc num :

NP_005498.1

ncbi gb acc num :

NM_005507.2

uniprot acc num :

P23528

ncbi mol weight :

18,502 Da

ncbi pathways :

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (1270303); BDNF Signaling Pathway (712093); CDC42 Signaling Events Pathway (137994); CXCR4-mediated Signaling Events Pathway (137910); Developmental Biology Pathway (1270302); EPH-Ephrin Signaling Pathway (1270330); EPHB-mediated Forward Signaling Pathway (1270332); Fc Gamma R-mediated Phagocytosis Pathway (114228)

ncbi summary :

The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]

uniprot summary :

Cofilin-1: a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues. Protein type: Nuclear receptor co-regulator; Motility/polarity/chemotaxis; Cytoskeletal. Chromosomal Location of Human Ortholog: 11q13. Cellular Component: cortical actin cytoskeleton; cytoplasm; extracellular space; focal adhesion; intercellular junction; membrane; nuclear matrix; nucleus; vesicle. Molecular Function: actin binding; protein binding. Biological Process: actin cytoskeleton organization and biogenesis; actin filament depolymerization; axon guidance; blood coagulation; cytokinesis after mitosis; cytoskeleton organization and biogenesis; ephrin receptor signaling pathway; establishment of cell polarity; innate immune response; negative regulation of apoptosis; neural crest cell migration; neural fold formation; platelet activation; platelet degranulation; positive regulation of actin filament depolymerization; protein amino acid phosphorylation; regulation of cell morphogenesis; response to amino acid stimulus; response to virus; Rho protein signal transduction

size1 :

48-Strip-Wells

price1 :

355 USD

size2 :

96-Strip-Wells

price2 :

475

size3 :

5x96-Strip-Wells

price3 :

2030

size4 :

10x96-Strip-Wells

price4 :

3700