ELISA/assay

Dog Telomerase reverse transcriptase ELISA Kit

MBS2886860

48-Strip-Wells

400 USD

ELISA Kit

Dog Telomerase reverse transcriptase ELISA Kit

[Telomerase reverse transcriptase]

[Telomerase reverse transcriptase; Telomerase catalytic subunit; TERT; 2.7.7.49]

[telomerase reverse transcriptase; Telomerase reverse transcriptase; telomerase reverse transcriptase; Telomerase catalytic subunit]

[TERT]

[TERT; TERT]

TERT_CANLF

Dog

1123

Recombinant and natural Dog Telomerase reverse transcriptase

For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids.

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

72255555

NP_001026800.1

NM_001031630.1

Q6A548

124,825 Da

Cell Cycle Pathway (1340367); Chromosome Maintenance Pathway (1340452); Extension Of Telomeres Pathway (1340456); Formation Of The Beta-catenin:TCF Transactivating Complex Pathway (1339815); HTLV-I Infection Pathway (373907); HTLV-I Infection Pathway (373889); IL-2 Signaling Pathway (198596); Id Signaling Pathway (198619); Signal Transduction Pathway (1339601); Signaling By Wnt Pathway (1339807)

Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis ().

48-Strip-Wells

400 USD

96-Strip-Wells

530

5x96-Strip-Wells

2305

10x96-Strip-Wells

4250