Human Tyrosine-protein kinase CSK ELISA Kit | MyBioSource MBS2886661 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Tyrosine-protein kinase CSK ELISA Kit

catalog :

MBS2886661

quantity :

48-Strip-Wells

price :

355 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2886661

products type :

ELISA Kit

products full name :

Human Tyrosine-protein kinase CSK ELISA Kit

products short name :

[Tyrosine-protein kinase CSK]

products name syn :

[Tyrosine-protein kinase CSK; C-Src kinase; Protein-tyrosine kinase CYL; CSK; 2.7.10.2]

other names :

[tyrosine-protein kinase CSK; Tyrosine-protein kinase CSK; tyrosine-protein kinase CSK; c-src tyrosine kinase; C-Src kinase; Protein-tyrosine kinase CYL]

products gene name :

[CSK]

other gene names :

[CSK; CSK]

uniprot entry name :

CSK_HUMAN

reactivity :

Human

sequence length :

450

specificity :

Recombinant and natural Mouse Semaphorin-3C

storage stability :

For long term storage, please store the entire kit at -20 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids. Detection Range: 15.6-1000 pg/mL

other info2 :

Intra-assay Precision: Intra-Assay CV: <=5.4%. Inter-assay Precision: Inter-Assay CV: <=7.9%

products description :

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

187475373

ncbi acc num :

NP_001120662.1

ncbi gb acc num :

NM_001127190.1

uniprot acc num :

P41240

ncbi mol weight :

50,704 Da

ncbi pathways :

ARMS-mediated Activation Pathway (1269471); Adaptive Immune System Pathway (1269171); Axon Guidance Pathway (1270303); B Cell Receptor Signaling Pathway (198909); BCR Signaling Pathway (138058); CXCR4-mediated Signaling Events Pathway (137910); Costimulation By The CD28 Family Pathway (1269177); Cytokine Signaling In Immune System Pathway (1269310); DAP12 Interactions Pathway (1269283); DAP12 Signaling Pathway (1269284)

uniprot summary :

CSK: a tyrosine kinase of the Csk family that phosphorylates and inhibits Src family kinases. Specifically phosphorylates Y504 on LCK, which acts as a negative regulatory site. Csk and the phospho-tyrosine phosphatase CD45 reciprocally regulate phosphorylation of the inhibitory tyrosine of the Src family kinases Lck and Fyn during T-cell receptor mediated signaling during thymic development. Protein type: EC 2.7.10.2; Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); TK group; Csk family. Chromosomal Location of Human Ortholog: 15q24.1. Cellular Component: cytoplasm; cytosol; extrinsic to internal side of plasma membrane; intercellular junction; lipid raft; plasma membrane. Molecular Function: ATP binding; identical protein binding; metal ion binding; non-membrane spanning protein tyrosine kinase activity; protein binding; protein C-terminus binding; protein phosphatase binding; protein-tyrosine kinase activity; receptor binding. Biological Process: adaptive immune response; blood coagulation; brain development; cell differentiation; cell migration; epidermal growth factor receptor signaling pathway; innate immune response; negative regulation of bone resorption; negative regulation of cell proliferation; negative regulation of Golgi to plasma membrane protein transport; negative regulation of interleukin-6 production; negative regulation of kinase activity; negative regulation of phagocytosis; oligodendrocyte differentiation; platelet activation; positive regulation of MAP kinase activity; protein amino acid phosphorylation; regulation of cell proliferation; regulation of cytokine production; T cell costimulation; T cell receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway

size1 :

48-Strip-Wells

price1 :

355 USD

size2 :

96-Strip-Wells

price2 :

475

size3 :

5x96-Strip-Wells

price3 :

2030

size4 :

10x96-Strip-Wells

price4 :

3700

more info or order :

MyBioSource product webpage