ELISA/assay

Pig High mobility group protein B1 ELISA Kit

MBS2886584

48-Strip-Wells

400 USD

ELISA Kit

Pig High mobility group protein B1 ELISA Kit

[High mobility group protein B1]

[High mobility group protein B1; High mobility group protein 1; HMG-1; HMGB1; HMG1]

[high mobility group protein B1; High mobility group protein B1; high mobility group protein B1; High mobility group protein 1; HMG-1]

[HMGB1]

[HMGB1; HMGB1; HMG1; HMG-1]

HMGB1_PIG

Pig

215

For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids.

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

51592117

NP_001004034.1

NM_001004034.1

P12682

24,917 Da

Activated TLR4 Signalling Pathway (1336942); Activation Of DNA Fragmentation Factor Pathway (1337141); Advanced Glycosylation Endproduct Receptor Signaling Pathway (1336960); Apoptosis Pathway (1337116); Apoptosis Induced DNA Fragmentation Pathway (1337140); Apoptotic Execution Phase Pathway (1337135); Base Excision Repair Pathway (92797); Base Excision Repair Pathway (451); Cytosolic Sensors Of Pathogen-associated DNA Pathway (1336968); DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway (1336976)

Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy. Acts as danger associated molecular pattern (DAMP) molecule that amplifies immune responses during tissue injury. Released to the extracellular environment can bind DNA, nucleosomes, IL-1 beta, CXCL12, AGER isoform 2/sRAGE, lipopolysaccharide (LPS) and lipoteichoic acid (LTA), and activates cells through engagement of multiple surface receptors. In the extracellular compartment fully reduced HMGB1 (released by necrosis) acts as a chemokine, disulfide HMGB1 (actively secreted) as a cytokine, and sulfonyl HMGB1 (released from apoptotic cells) promotes immunological tolerance. Has proangiogenic activity. May be involved in platelet activation. Binds to phosphatidylserine and phosphatidylethanolamide. Bound to RAGE mediates signaling for neuronal outgrowth. May play a role in accumulation of expanded polyglutamine (polyQ) proteins ().

48-Strip-Wells

400 USD

96-Strip-Wells

530

5x96-Strip-Wells

2305

10x96-Strip-Wells

4250