Human Klotho ELISA Kit | MyBioSource MBS2886510 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Klotho ELISA Kit

catalog :

MBS2886510

quantity :

48-Strip-Wells

price :

355 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2886510

products type :

ELISA Kit

products full name :

Human Klotho ELISA Kit

products short name :

[Klotho]

products name syn :

[Klotho; KL; 3.2.1.31]

other names :

[klotho; Klotho; klotho; klotho]

products gene name :

[KL]

other gene names :

[KL; KL]

uniprot entry name :

KLOT_HUMAN

reactivity :

Human

sequence length :

1012

storage stability :

For long term storage, please store the entire kit at -20 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids.

products description :

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

24497614

ncbi acc num :

NP_004786.2

ncbi gb acc num :

NM_004795.3

uniprot acc num :

Q9UEF7

ncbi mol weight :

62,135 Da

ncbi pathways :

ARMS-mediated Activation Pathway (1269471); Adaptive Immune System Pathway (1269171); Axon Guidance Pathway (1270303); Constitutive Signaling By Aberrant PI3K In Cancer Pathway (1268880); Cytokine Signaling In Immune System Pathway (1269310); DAP12 Interactions Pathway (1269283); DAP12 Signaling Pathway (1269284); Developmental Biology Pathway (1270302); Disease Pathway (1268854); Diseases Of Signal Transduction Pathway (1268855)

ncbi summary :

This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss. [provided by RefSeq, Jul 2008]

uniprot summary :

Klotho: May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1. Defects in KL are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC). A severe metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Some patients manifest recurrent, transient, painful swellings of the long bones associated with the radiographic findings of periosteal reaction and cortical hyperostosis and absence of skin involvement. Belongs to the glycosyl hydrolase 1 family. Klotho subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.2.1.31; Membrane protein, integral; Hydrolase. Chromosomal Location of Human Ortholog: 13q12. Cellular Component: apical plasma membrane; extracellular region; extracellular space; integral to membrane; integral to plasma membrane; intracellular; plasma membrane. Molecular Function: beta-glucosidase activity; beta-glucuronidase activity; fibroblast growth factor binding; fibroblast growth factor receptor binding; hormone activity; signal transducer activity; vitamin D binding. Biological Process: activation of MAPKK activity; aging; axon guidance; calcium ion homeostasis; carbohydrate metabolic process; energy reserve metabolic process; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; innate immune response; insulin receptor signaling pathway; MAPKKK cascade; nerve growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of bone mineralization; Ras protein signal transduction; small GTPase mediated signal transduction; vascular endothelial growth factor receptor signaling pathway. Disease: Tumoral Calcinosis, Hyperphosphatemic, Familial

size1 :

48-Strip-Wells

price1 :

355 USD

size2 :

96-Strip-Wells

price2 :

475

size3 :

5x96-Strip-Wells

price3 :

2030

size4 :

10x96-Strip-Wells

price4 :

3700

more info or order :

MyBioSource product webpage