catalog number :
MBS2886354
products type :
ELISA Kit
products full name :
Rat Glial fibrillary acidic protein ELISA Kit
products short name :
[Glial fibrillary acidic protein]
products name syn :
[Glial fibrillary acidic protein; GFAP; Gfap]
other names :
[glial fibrillary acidic protein; Glial fibrillary acidic protein; glial fibrillary acidic protein; glial fibrillary acidic protein]
products gene name :
[Gfap]
other gene names :
[Gfap; Gfap; GFAP]
uniprot entry name :
GFAP_RAT
storage stability :
For long term storage, please store the entire kit at -20 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Assay Type: Sandwich. Detection Range: 31.2-2000 pg/mL. Sensitivity: 7.8 pg/mL
other info2 :
Intra-Assay CV: <=5.3%. Inter-Assay CV: <=10.2%. Recovery: 97%
products description :
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_058705.2
ncbi gb acc num :
NM_017009.2
ncbi mol weight :
48,782 Da
ncbi pathways :
Jak-STAT Signaling Pathway (83469); Jak-STAT Signaling Pathway (488); Nuclear Signaling By ERBB4 Pathway (1333894); Signal Transduction Pathway (1333779); Signaling By ERBB4 Pathway (1333891); Spinal Cord Injury Pathway (739006)
ncbi summary :
intermediate filament protein specific for astrocytes [RGD, Feb 2006]
uniprot summary :
GFAP: a class-III intermediate filament protein. A cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant isoform has been described, but its full length sequence has not been determined. Protein type: Cytoskeletal. Cellular Component: cell projection; cytoplasm; intermediate filament; intermediate filament cytoskeleton; intracellular; membrane; myelin sheath. Molecular Function: glycoprotein binding; integrin binding; kinase binding; structural constituent of cytoskeleton; structural molecule activity. Biological Process: astrocyte development; Bergmann glial cell differentiation; extracellular matrix organization and biogenesis; intermediate filament organization; intermediate filament-based process; neurite regeneration; regulation of neurotransmitter uptake; response to wounding
size4 :
10x96-Strip-Wells