Human D-amino-acid oxidase ELISA Kit | MyBioSource MBS2886321 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human D-amino-acid oxidase ELISA Kit

catalog :

MBS2886321

quantity :

48-Strip-Wells

price :

355 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2886321

products type :

ELISA Kit

products full name :

Human D-amino-acid oxidase ELISA Kit

products short name :

[D-amino-acid oxidase]

products name syn :

[D-amino-acid oxidase; DAAO; DAMOX; DAO; DAO; DAMOX; 1.4.3.3]

other names :

[D-amino-acid oxidase; D-amino-acid oxidase; D-amino-acid oxidase; D-amino-acid oxidase]

products gene name :

[DAO]

other gene names :

[DAO; DAO; DAAO; OXDA; DAMOX; DAMOX; DAAO; DAMOX; DAO]

uniprot entry name :

OXDA_HUMAN

reactivity :

Human

sequence length :

347

specificity :

Recombinant and natural Human D-amino-acid oxidase,DAAO

storage stability :

For long term storage, please store the entire kit at -20 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Detection Range: 78-5000 pg/mL. Sensitivity: <38 pg/mL

other info2 :

Intra-assay Precision: Intra-Assay CV: <=5.9%. Inter-assay Precision: Inter-Assay CV: <=9.1%

products description :

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

148539837

ncbi acc num :

NP_001908.3

ncbi gb acc num :

NM_001917.4

uniprot acc num :

P14920

ncbi mol weight :

39,474 Da

ncbi pathways :

Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); D-Arginine And D-ornithine Metabolism Pathway (82972); D-Arginine And D-ornithine Metabolism Pathway (341); Glycine, Serine And Threonine Metabolism Pathway (82949); Glycine, Serine And Threonine Metabolism Pathway (313); Glyoxylate Metabolism And Glycine Degradation Pathway (1270180); Metabolic Pathways (132956); Metabolism Pathway (1269956); Metabolism Of Amino Acids And Derivatives Pathway (1270158)

ncbi summary :

This gene encodes the peroxisomal enzyme D-amino acid oxidase. The enzyme is a flavoprotein which uses flavin adenine dinucleotide (FAD) as its prosthetic group. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. Its biological function is not known; it may act as a detoxifying agent which removes D-amino acids that accumulate during aging. In mice, it degrades D-serine, a co-agonist of the NMDA receptor. This gene may play a role in the pathophysiology of schizophrenia. [provided by RefSeq, Jul 2008]

uniprot summary :

DAO: Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D- amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids. Belongs to the DAMOX/DASOX family. Protein type: Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - glycine, serine and threonine; EC 1.4.3.3; Oxidoreductase; Other Amino Acids Metabolism - D-Arginine and D-ornithine. Chromosomal Location of Human Ortholog: 12q24. Cellular Component: cytosol; mitochondrial outer membrane; peroxisomal matrix; peroxisomal membrane; peroxisome. Molecular Function: cofactor binding; D-amino-acid oxidase activity; protein binding; protein dimerization activity; receptor binding. Biological Process: dopamine biosynthetic process; glyoxylate metabolic process; proline catabolic process. Disease: Schizophrenia

size1 :

48-Strip-Wells

price1 :

355 USD

size2 :

96-Strip-Wells

price2 :

475

size3 :

5x96-Strip-Wells

price3 :

2030

size4 :

10x96-Strip-Wells

price4 :

3700

more info or order :

MyBioSource product webpage