Rat Apolipoprotein B-100 ELISA Kit | MyBioSource MBS2886246 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Apolipoprotein B-100 ELISA Kit

catalog :

MBS2886246

quantity :

48-Strip-Wells

price :

380 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2886246

products type :

ELISA Kit

products full name :

Rat Apolipoprotein B-100 ELISA Kit

products short name :

Apolipoprotein B-100

products name syn :

Apolipoprotein B-100; Apo B-100; Apob; Aa1064; Ac1-060

other names :

apolipoprotein B-100; Apolipoprotein B-100; apolipoprotein B-100; apolipoprotein B

products gene name :

Apob

other gene names :

Apob; Apob; Aa1064; Ac1-060; ApoB-48; ApoB-100; Apo B-100; Apo B-100; Apo B-48

uniprot entry name :

APOB_RAT

reactivity :

Rat

sequence length :

4506

storage stability :

For long term storage, please store the entire kit at -20 degree C.

other info1 :

Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

products description :

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

161783809

ncbi acc num :

NP_062160.2

ncbi gb acc num :

NM_019287.2

uniprot acc num :

Q7TMA5

ncbi mol weight :

536,024 Da

ncbi pathways :

Binding And Uptake Of Ligands By Scavenger Receptors Pathway (1333660); Cell Surface Interactions At The Vascular Wall Pathway (1333067); Chylomicron-mediated Lipid Transport Pathway (1333315); Fat Digestion And Absorption Pathway (194376); Fat Digestion And Absorption Pathway (194324); Hemostasis Pathway (1333034); LDL-mediated Lipid Transport Pathway (1333317); Lipid Digestion, Mobilization, And Transport Pathway (1333311); Lipoprotein Metabolism Pathway (1333314); Metabolism Pathway (1333271)

ncbi summary :

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-L and apoB-H. Unlike the apoB-48 and apoB-100 structural equivalents in human, which are synthesized exclusively in the gut and liver, respectively, the rat apoBL isoform is also found in rat liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-L protein is produced after RNA editing of the apoB-H transcript at residue 2180 (CAA- UAA), resulting in the creation of a stop codon, and early translation termination. [provided by RefSeq, Jul 2008]

uniprot summary :

APOB: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. Defects in APOB are a cause of familial hypobetalipoproteinemia type 1 (FHBL1). A disorder characterized by highly reduced plasma concentrations of low density lipoproteins, and dietary fat malabsorption. Clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia. Defects in APOB are a cause of familial ligand-defective apolipoprotein B-100 (FDB). FDB is a dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors. Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia. Protein type: Secreted; Carrier; Secreted, signal peptide. Cellular Component: actin cytoskeleton; cell soma; chylomicron; cytoplasm; endoplasmic reticulum; extracellular space; Golgi apparatus; intracellular membrane-bound organelle; plasma membrane; vesicle membrane. Molecular Function: cholesterol transporter activity; heparin binding; lipid binding; lipid transporter activity; low-density lipoprotein receptor binding; phospholipid binding; protein binding. Biological Process: artery morphogenesis; cholesterol efflux; cholesterol homeostasis; cholesterol metabolic process; cholesterol transport; fertilization; in utero embryonic development; lipid catabolic process; lipid metabolic process; lipid transport; lipoprotein biosynthetic process; lipoprotein catabolic process; lipoprotein metabolic process; lipoprotein transport; nervous system development; post-embryonic development; regulation of cholesterol biosynthetic process; response to carbohydrate stimulus; response to lipopolysaccharide; response to organic substance; response to selenium ion; response to virus; sperm motility; spermatogenesis; triacylglycerol catabolic process; triacylglycerol mobilization

size1 :

48-Strip-Wells

price1 :

380 USD

size2 :

96-Strip-Wells

price2 :

500

more info or order :

MyBioSource product webpage