ELISA/assay

Mouse Apoptosis-associated speck-like protein containing a CARD ELISA Kit

MBS2885479

48-Strip-Wells

355 USD

ELISA Kit

Mouse Apoptosis-associated speck-like protein containing a CARD ELISA Kit

[Apoptosis-associated speck-like protein containing a CARD]

[Apoptosis-associated speck-like protein containing a CARD; mASC; PYD and CARD domain-containing protein; Pycard; Asc]

[apoptosis-associated speck-like protein containing a CARD; Apoptosis-associated speck-like protein containing a CARD; apoptosis-associated speck-like protein containing a CARD; PYD and CARD domain containing; PYD and CARD domain-containing protein]

[Pycard]

[Pycard; Pycard; Asc; TNS1; masc; CARD5; TMS-1; 9130417A21Rik; Asc; mASC]

ASC_MOUSE

Mouse

193

Recombinant and natural Human GDH 1

For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids. Detection Range: 0.156-10 ng/mL. Sensitivity: <0.1 ng/mL

Intra-assay Precision: Intra-Assay CV: <=4.1%. Inter-assay Precision: Inter-Assay CV: <=6.5%

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

165377185

NP_075747.3

NM_023258.4

Q9EPB4

21,459 Da

C-type Lectin Receptors (CLRs) Pathway (1323757); CLEC7A (Dectin-1) Signaling Pathway (1323758); CLEC7A/inflammasome Pathway (1323761); Cytosolic DNA-sensing Pathway (125146); Cytosolic DNA-sensing Pathway (124833); Immune System Pathway (1323639); Inflammasomes Pathway (1323718); Influenza A Pathway (217174); Influenza A Pathway (217150); Innate Immune System Pathway (1323671)

PYCARD: Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. Forms complexes with other DAPIN domain-containing proteins. Interacts with CIAS1/PYPAF1 and PYDC1. Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U-937, but not in Jurkat T- cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and MOLT-4 lymphocytic leukemia cells. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Tumor suppressor; Adaptor/scaffold. Cellular Component: cell soma; cytoplasm; cytosol; endoplasmic reticulum; extracellular region; IkappaB kinase complex; mitochondrion; nucleolus; nucleus; protein complex. Molecular Function: apoptotic protease activator activity; enzyme binding; identical protein binding; interleukin-6 receptor binding; myosin I binding; protease binding; protein binding; protein dimerization activity; protein homodimerization activity; Pyrin domain binding; tropomyosin binding. Biological Process: activation of innate immune response; activation of NF-kappaB transcription factor; apoptosis; caspase activation; defense response to Gram-negative bacterium; defense response to virus; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; immune system process; inflammatory response; inhibition of NF-kappaB transcription factor; innate immune response; interleukin-1 beta production; myeloid dendritic cell activation; myeloid dendritic cell activation during immune response; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of interferon-beta production; positive regulation of actin filament polymerization; positive regulation of activated T cell proliferation; positive regulation of adaptive immune response; positive regulation of antigen processing and presentation of peptide antigen via MHC class II; positive regulation of apoptosis; positive regulation of caspase activity; positive regulation of defense response to virus by host; positive regulation of interferon-gamma production; positive regulation of interleukin-1 beta secretion; positive regulation of interleukin-6 production; positive regulation of JNK cascade; positive regulation of phagocytosis; positive regulation of T cell activation; positive regulation of transcription factor activity; positive regulation of tumor necrosis factor production; regulation of apoptosis; regulation of autophagy; regulation of caspase activity; regulation of GTPase activity; regulation of inflammatory response; regulation of protein stability; response to bacterium; tumor necrosis factor-mediated signaling pathway

48-Strip-Wells

355 USD

96-Strip-Wells

475

5x96-Strip-Wells

2030

10x96-Strip-Wells

3700