catalog number :
MBS2885379
products type :
ELISA Kit
products full name :
Mouse Tryptase ELISA Kit
products short name :
[Tryptase]
products name syn :
[Tryptase; Mast cell protease 7; mMCP-7; Tryptase alpha/beta-1; Tpsab1; Mcpt7; 3.4.21.59]
other names :
[tryptase; Tryptase; tryptase; tryptase alpha/beta 1; Mast cell protease 7; mMCP-7; Tryptase alpha/beta-1]
products gene name :
[Tpsab1]
other gene names :
[Tpsab1; Tpsab1; Mcp7; Mcp-7; Mcpt7; MMCP-7; Mcpt7; mMCP-7]
uniprot entry name :
TRYB1_MOUSE
storage stability :
For long term storage, please store the entire kit at -20 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Assay Type: Sandwich. Detection Range: 0.78-50 ng/mL. Sensitivity: 0.398 ng/mL
other info2 :
Intra-Assay CV: <=5.5%. Inter-Assay CV: <=8.6%. Recovery: 100%
products description :
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_112464.4
ncbi gb acc num :
NM_031187.4
ncbi mol weight :
4,896 Da
uniprot summary :
TPSAB1: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Belongs to the peptidase S1 family. Tryptase subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide; Protease; EC 3.4.21.59
size4 :
10x96-Strip-Wells