ELISA/assay

Human Gastrin-releasing peptide ELISA Kit

MBS2885375

48-Strip-Wells

400 USD

ELISA Kit

Human Gastrin-releasing peptide ELISA Kit

[Gastrin-releasing peptide]

[Gastrin-releasing peptide; GRP; GRP]

[gastrin-releasing peptide isoform 2; Gastrin-releasing peptide; gastrin-releasing peptide; gastrin releasing peptide; Neuromedin-CAlternative name(s):GRP-10]

[GRP]

[GRP; GRP; BN; GRP-10; proGRP; preproGRP; GRP]

GRP_HUMAN

Human

141

For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Assay Type: Competitive. Detection Range: 0.156-10 ng/mL. Sensitivity: 0.057ng/ml

Intra-Assay CV: <=7.1. Inter-Assay CV: <=9.3. Recovery: 102%

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen, During the reaction, target antigen in the sample or standard competes with a fixed amount of biotin-labeled target antigen for sites on a pre-coated antibody specific to target antigen. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

60498997

NP_001012530.1

NM_001012512.2

P07492

15,533 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (1269545); G Alpha (q) Signalling Events Pathway (1269578); GPCR Downstream Signaling Pathway (1269574); GPCR Ligand Binding Pathway (1269544); Gastrin-CREB Signalling Pathway Via PKC And MAPK (1269592); Incretin Synthesis, Secretion, And Inactivation Pathway (1268750); Metabolism Of Proteins Pathway (1268677); Peptide Hormone Metabolism Pathway (1268746); Peptide Ligand-binding Receptors Pathway (1269546); Signal Transduction Pathway (1269379)

This gene encodes a member of the bombesin-like family of gastrin-releasing peptides. The encoded preproprotein is proteolytically processed to generate two peptides, gastrin-releasing peptide and neuromedin-C. These peptides regulate numerous functions of the gastrointestinal and central nervous systems, including release of gastrointestinal hormones, smooth muscle cell contraction, and epithelial cell proliferation. These peptides are also likely to play a role in human cancers of the lung, colon, stomach, pancreas, breast, and prostate. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]

GRP: GRP stimulates gastrin release as well as other gastrointestinal hormones. Operates as a negative feedback regulating fear and established a causal relationship between GRP- receptor gene expression, long-term potentiation, and amygdala- dependent memory for fear. Belongs to the bombesin/neuromedin-B/ranatensin family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 18q21.1-q21.32. Cellular Component: extracellular region; extracellular space. Molecular Function: neuropeptide hormone activity; receptor binding. Biological Process: neuropeptide signaling pathway; signal transduction

48-Strip-Wells

400 USD

96-Strip-Wells

530

5x96-Strip-Wells

2305

10x96-Strip-Wells

4250