Human Osteocalcin ELISA Kit | MyBioSource MBS2885169 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Osteocalcin ELISA Kit

catalog :

MBS2885169

quantity :

48-Strip-Wells

price :

355 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2885169

products type :

ELISA Kit

products full name :

Human Osteocalcin ELISA Kit

products short name :

[Osteocalcin]

products name syn :

[Osteocalcin; Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein; BGLAP]

other names :

[osteocalcin preproprotein; Osteocalcin; osteocalcin; bone gamma-carboxyglutamate protein; Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein]

products gene name :

[BGLAP]

other gene names :

[BGLAP; BGLAP; OC; BGP; OCN; BGP]

uniprot entry name :

OSTCN_HUMAN

reactivity :

Human

sequence length :

100

specificity :

Recombinant and natural Mouse PPIase FKBP5

storage stability :

For long term storage, please store the entire kit at -20 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids. Detection Range: 0.156-10 ng/mL. Sensitivity: <0.063 ng/mL

other info2 :

Intra-assay Precision: Intra-Assay CV: <=7.1%. Inter-assay Precision: Inter-Assay CV: <=10.5%

products description :

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

40316933

ncbi acc num :

NP_954642.1

ncbi gb acc num :

NM_199173.5

uniprot acc num :

P02818

ncbi mol weight :

10,963 Da

ncbi pathways :

FGF Signaling Pathway (137989); Gamma Carboxylation, Hypusine Formation And Arylsulfatase Activation Pathway (1268702); Gamma-carboxylation Of Protein Precursors Pathway (1268704); Gamma-carboxylation, Transport, And Amino-terminal Cleavage Of Proteins Pathway (1268703); Glucocorticoid Receptor Regulatory Network Pathway (138014); Interleukin-11 Signaling Pathway (698753); Metabolism Of Proteins Pathway (1268677); Notch-mediated HES/HEY Network Pathway (169347); Post-translational Protein Modification Pathway (1268701); Regulation Of Retinoblastoma Protein Pathway (137916)

ncbi summary :

This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product. [provided by RefSeq, Jun 2015]

uniprot summary :

osteocalcin: Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Belongs to the osteocalcin/matrix Gla protein family. Protein type: Cell development/differentiation; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1q22. Cellular Component: cytoplasm; dendrite; endoplasmic reticulum lumen; extracellular space; Golgi lumen; perikaryon; rough endoplasmic reticulum. Molecular Function: calcium ion binding; hydroxyapatite binding; structural constituent of bone; structural molecule activity. Biological Process: bone mineralization; cell adhesion; cell aging; cellular protein metabolic process; ER to Golgi vesicle-mediated transport; odontogenesis; osteoblast development; osteoblast differentiation; peptidyl-glutamic acid carboxylation; post-translational protein modification; regulation of bone mineralization; regulation of bone resorption; regulation of osteoclast differentiation; response to activity; response to drug; response to estrogen stimulus; response to ethanol; response to glucocorticoid stimulus; response to gravity; response to hydroxyisoflavone; response to mechanical stimulus; response to testosterone stimulus; response to vitamin D; response to vitamin K; response to zinc ion; signal peptide processing; skeletal development

size1 :

48-Strip-Wells

price1 :

355 USD

size2 :

96-Strip-Wells

price2 :

475

size3 :

5x96-Strip-Wells

price3 :

2030

size4 :

10x96-Strip-Wells

price4 :

3700

more info or order :

MyBioSource product webpage