ELISA/assay

Rat Complement C5 ELISA Kit

MBS2885017

48-Strip-Wells

355 USD

ELISA Kit

Rat Complement C5 ELISA Kit

[Complement C5]

[Complement C5; C5]

[Complement C5; Complement C5; complement C5; complement component 5]

[C5]

[C5; C5; Hc; C5a; RGD1561905]

CO5_RAT

Rat

77

For long term storage, please store the entire kit at -20 degree C.

Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids.

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

46397770

P08650.2

P08650

8,981 Da

Activation Of C3 And C5 Pathway (1332828); Class A/1 (Rhodopsin-like Receptors) Pathway (1333935); Complement Activation, Classical Pathway (198509); Complement And Coagulation Cascades Pathway (83465); Complement And Coagulation Cascades Pathway (484); Complement Cascade Pathway (1332823); G Alpha (i) Signalling Events Pathway (1333966); GPCR Downstream Signaling Pathway (1333964); GPCR Ligand Binding Pathway (1333934); Herpes Simplex Infection Pathway (377869)

likely component of the classical pathway of the complement cascade; may be involved with tissue injury associated with ischemia or sepsis; may be a component of the membrane attack complex [RGD, Feb 2006]

C5: Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. Defects in C5 are the cause of complement component 5 deficiency (C5D). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705). Protein type: Secreted; Secreted, signal peptide. Cellular Component: cell; extracellular space. Molecular Function: C5a anaphylatoxin chemotactic receptor binding. Biological Process: cellular calcium ion homeostasis; chemotaxis; complement activation, alternative pathway; complement activation, classical pathway; glucose homeostasis; in utero embryonic development; inflammatory response; leukocyte migration during inflammatory response; negative regulation of dopamine secretion; positive regulation of angiogenesis; positive regulation of chemotaxis

48-Strip-Wells

355 USD

96-Strip-Wells

475

5x96-Strip-Wells

2030

10x96-Strip-Wells

3700