ELISA/assay

Rat Nitric oxide synthase, brain ELISA Kit

MBS2884948

48-Strip-Wells

305 USD

ELISA Kit

Rat Nitric oxide synthase, brain ELISA Kit

[Nitric oxide synthase, brain]

[Nitric oxide synthase, brain; BNOS; Constitutive NOS; NC-NOS; NOS type I; Neuronal NOS; N-NOS; nNOS; Peptidyl-cysteine S-nitrosylase NOS1; Nos1; Bnos; 1.14.13.39]

[nitric oxide synthase, brain; Nitric oxide synthase, brain; nitric oxide synthase, brain; nitric oxide synthase 1; BNOS; Constitutive NOS; NC-NOS; NOS type I; Neuronal NOS; N-NOS; nNOS; Peptidyl-cysteine S-nitrosylase NOS1]

[Nos1]

[Nos1; Nos1; bNOS; Bnos; N-NOS; nNOS]

NOS1_RAT

Rat

1463

Recombinant and natural Mouse Leptin

For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids. Detection Range: 0.312-20 ng/mL

Intra-assay Precision: Intra-Assay CV: <=5.7%. Inter-assay Precision: Inter-Assay CV: <=7.9%

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

16258811

NP_434686.1

NM_052799.1

P29476

164,430 Da

Alzheimer's Disease Pathway (83489); Alzheimer's Disease Pathway (509); Amyotrophic Lateral Sclerosis (ALS) Pathway (83491); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Arginine And Proline Metabolism Pathway (83349); Arginine And Proline Metabolism Pathway (323); Arginine Biosynthesis Pathway (83336); Arginine Biosynthesis Pathway (306); Calcium Signaling Pathway (83442); Calcium Signaling Pathway (459)

enzyme that catalyzes the production of nitric oxide [RGD, Feb 2006]

nNOS: nitric oxide synthase 1, neuronal form. Produces nitric oxide which is a messenger molecule with diverse functions and displays many properties of a neurotransmitter in the brain and peripheral nervous system. May be an effector enzyme for the dystrophin complex. Four alternatively spliced isoforms have been described. Protein type: EC 1.14.13.39; Amino Acid Metabolism - arginine and proline; Oxidoreductase. Cellular Component: azurophil granule; caveola; cytoplasm; cytoskeleton; cytosol; dendrite; dendritic spine; lipid raft; membrane; mitochondrial outer membrane; mitochondrion; nuclear membrane; nucleus; perinuclear region of cytoplasm; photoreceptor inner segment; plasma membrane; postsynaptic density; protein complex; sarcolemma; sarcoplasmic reticulum; sarcoplasmic reticulum membrane; synapse; T-tubule; vesicle membrane; Z disc. Molecular Function: amino acid binding; ATPase binding; cadmium ion binding; calmodulin binding; enzyme binding; FAD binding; FMN binding; heme binding; iron ion binding; NADP binding; nitric-oxide synthase activity; protein binding; protein homodimerization activity; sodium channel regulator activity. Biological Process: aging; arginine catabolic process; behavioral response to cocaine; exogenous drug catabolic process; female pregnancy; multicellular organismal response to stress; negative regulation of apoptosis; negative regulation of blood pressure; negative regulation of calcium ion transport; negative regulation of cell proliferation; negative regulation of heart contraction; negative regulation of hydrolase activity; negative regulation of insulin secretion; negative regulation of peptidyl-serine phosphorylation; negative regulation of potassium ion transport; negative regulation of serotonin uptake; negative regulation of vasoconstriction; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; peptidyl-cysteine S-nitrosylation; positive regulation of guanylate cyclase activity; positive regulation of histone acetylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of vasodilation; reduction of cytosolic calcium ion concentration; regulation of heart contraction; regulation of neurogenesis; regulation of sensory perception of pain; regulation of sodium ion transport; response to activity; response to estrogen stimulus; response to ethanol; response to heat; response to hypoxia; response to lead ion; response to lipopolysaccharide; response to nicotine; response to nutrient levels; response to organic cyclic substance; response to organic nitrogen; response to peptide hormone stimulus; response to vitamin E; striated muscle contraction

48-Strip-Wells

305 USD

96-Strip-Wells

395

5x96-Strip-Wells

1615

10x96-Strip-Wells

2880