ELISA/assay

Mouse Annexin A1 ELISA Kit

MBS2884661

48-Strip-Wells

355 USD

ELISA Kit

Mouse Annexin A1 ELISA Kit

[Annexin A1]

[Annexin A1; Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; Phospholipase A2 inhibitory protein; p35; Anxa1; Anx1; Lpc-1; Lpc1]

[annexin A1; Annexin A1; annexin A1; annexin A1; Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I]

[Anxa1]

[Anxa1; Anxa1; Lpc1; Anx-1; Lpc-1; Anx-A1; C430014K04Rik; Anx1; Lpc-1; Lpc1]

ANXA1_MOUSE

Mouse

346

Recombinant and natural Bovine Cathepsin L1

For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids. Detection Range: 0.78-50 ng/mL

Intra-assay Precision: Intra-Assay CV: <=5.7%. Inter-assay Precision: Inter-Assay CV: <=8.8%

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

124517663

NP_034860.2

NM_010730.2

P10107

38,734 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (1324706); Formyl Peptide Receptors Bind Formyl Peptides And Many Other Ligands Pathway (1324712); G Alpha (i) Signalling Events Pathway (1324737); G Alpha (q) Signalling Events Pathway (1324739); GPCR Downstream Signaling Pathway (1324735); GPCR Ligand Binding Pathway (1324705); Gastrin-CREB Signalling Pathway Via PKC And MAPK (1324753); Peptide Ligand-binding Receptors Pathway (1324707); Prostaglandin Synthesis And Regulation Pathway (198286); Signal Transduction Pathway (1324550)

ANXA1: a calcium/phospholipid-binding protein with which promotes membrane fusion and is involved in endocytosis. Has anti-inflammatory properties and inhibits phospholipase A2 activity. Accumulates on internalized vesicles after EGF-stimulated endocytosis, suggesting that it may be required for a late stage in inward vesiculation. Phosphorylated by PKC, EGFR and Chak1. Phosphorylation results in loss of the inhibitory activity. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion. Protein type: Lipid-binding; Calcium-binding. Cellular Component: apical plasma membrane; cell projection; cell surface; cilium; cornified envelope; cytoplasm; cytoplasmic vesicle; endosome; extracellular region; extracellular space; extrinsic to external side of plasma membrane; extrinsic to membrane; focal adhesion; lateral plasma membrane; mast cell granule; membrane; mitochondrial membrane; nucleoplasm; nucleus; plasma membrane; protein complex; sarcolemma; vesicle. Molecular Function: calcium ion binding; calcium-dependent phospholipid binding; calcium-dependent protein binding; double-stranded DNA-dependent ATPase activity; helicase activity; metal ion binding; phospholipase A2 inhibitor activity; phospholipase inhibitor activity; phospholipid binding; protein binding; protein binding, bridging; protein homodimerization activity; single-stranded DNA binding; single-stranded RNA binding; structural molecule activity. Biological Process: actin cytoskeleton reorganization; adaptive immune response; alpha-beta T cell differentiation; arachidonic acid secretion; cell surface receptor linked signal transduction; DNA duplex unwinding; DNA strand renaturation; G-protein signaling, coupled to cyclic nucleotide second messenger; immune system process; inflammatory response; innate immune response; insulin secretion; keratinocyte differentiation; monocyte chemotaxis; myoblast migration involved in skeletal muscle regeneration; negative regulation of exocytosis; negative regulation of protein secretion; negative regulation of T-helper 2 cell differentiation; neutrophil homeostasis; peptide cross-linking; phagocytosis; positive regulation of apoptosis; positive regulation of interleukin-2 production; positive regulation of neutrophil apoptosis; positive regulation of prostaglandin biosynthetic process; positive regulation of T cell proliferation; positive regulation of T-helper 1 cell differentiation; positive regulation of vesicle fusion; regulation of cell proliferation; regulation of cell shape; regulation of hormone secretion; regulation of inflammatory response; regulation of interleukin-1 production; regulation of leukocyte migration; signal transduction

48-Strip-Wells

355 USD

96-Strip-Wells

475

5x96-Strip-Wells

2030

10x96-Strip-Wells

3700