ELISA/assay

Human Protein Wnt-3a ELISA Kit

MBS2884635

48-Strip-Wells

355 USD

ELISA Kit

Human Protein Wnt-3a ELISA Kit

[Protein Wnt-3a]

[Protein Wnt-3a; WNT3A]

[protein Wnt-3a; Protein Wnt-3a; protein Wnt-3a; wingless-type MMTV integration site family member 3A]

[WNT3A]

[WNT3A; WNT3A]

WNT3A_HUMAN

Human

352

Recombinant and natural Mouse PEDF

For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids. Detection Range: 0.78-50 ng/mL

Intra-assay Precision: Intra-Assay CV: <=5.3%. Inter-assay Precision: Inter-Assay CV: <=9.9%

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

14916475

NP_149122.1

NM_033131.3

P56704

42,905 Da

Basal Cell Carcinoma Pathway (83113); Basal Cell Carcinoma Pathway (525); Canonical Wnt Signaling Pathway (138032); Cardiac Progenitor Differentiation Pathway (712094); Class B/2 (Secretin Family Receptors) Pathway (1269570); DNA Damage Response (only ATM Dependent) Pathway (198827); Disassembly Of The Destruction Complex And Recruitment Of AXIN To The Membrane Pathway (1269601); Disease Pathway (1268854); Diseases Of Signal Transduction Pathway (1268855); GPCR Ligand Binding Pathway (1269544)

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. [provided by RefSeq, Jul 2008]

WNT3A: Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. Interacts with PORCN. Interacts with APCDD1 and WLS. Component of the Wnt-Fzd-LRP5-LRP6 signaling complex that contains a WNT protein, a FZD protein and LRP5 or LRP6. Interacts directly in the complex with LRP6. Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate. Belongs to the Wnt family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 1q42. Cellular Component: cell surface; early endosome membrane; endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi lumen; plasma membrane; proteinaceous extracellular matrix. Molecular Function: frizzled binding; frizzled-2 binding; protein binding; protein domain specific binding; receptor agonist activity; receptor binding; transcription coactivator activity. Biological Process: axon guidance; cell proliferation in forebrain; cell proliferation in midbrain; dorsoventral neural tube patterning; extracellular matrix organization and biogenesis; heart looping; hemopoiesis; hippocampus development; in utero embryonic development; inner ear morphogenesis; mammary gland development; negative regulation of axon extension involved in axon guidance; negative regulation of fat cell differentiation; negative regulation of neurogenesis; neuron differentiation; osteoblast differentiation; palate development; paraxial mesodermal cell fate commitment; positive regulation of B cell proliferation; positive regulation of caspase activity; positive regulation of cell proliferation; positive regulation of collateral sprouting in the absence of injury; positive regulation of cytokine production; positive regulation of mesodermal cell fate specification; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of protein binding; positive regulation of receptor internalization; positive regulation of skeletal muscle development; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; signalosome assembly; spinal cord association neuron differentiation; synaptogenesis; Wnt receptor signaling pathway in forebrain neuroblast division; Wnt receptor signaling pathway through beta-catenin

48-Strip-Wells

355 USD

96-Strip-Wells

475

5x96-Strip-Wells

2030

10x96-Strip-Wells

3700