ELISA/assay

Human Peroxisome proliferator-activated receptor gamma coactivator 1-alpha ELISA Kit

MBS2884447

48-Strip-Wells

355 USD

ELISA Kit

Human Peroxisome proliferator-activated receptor gamma coactivator 1-alpha ELISA Kit

[Peroxisome proliferator-activated receptor gamma coactivator 1-alpha]

[Peroxisome proliferator-activated receptor gamma coactivator 1-alpha; PGC-1-alpha; PPAR-gamma coactivator 1-alpha; PPARGC-1-alpha; Ligand effect modulator 6; PPARGC1A; LEM6; PGC1]

[peroxisome proliferator-activated receptor gamma coactivator 1-alpha; Peroxisome proliferator-activated receptor gamma coactivator 1-alpha; peroxisome proliferator-activated receptor gamma coactivator 1-alpha; PPARG coactivator 1 alpha; Ligand effect modulator 6]

[PPARGC1A]

[PPARGC1A; PPARGC1A; LEM6; PGC1; PGC1A; PGC-1v; PPARGC1; PGC-1alpha; PGC-1(alpha); LEM6; PGC1; PGC1A; PPARGC1; PGC-1-alpha; PPAR-gamma coactivator 1-alpha; PPARGC-1-alpha]

PRGC1_HUMAN

Human

798

For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Assay Type: Sandwich. Detection Range: 0.312-20 ng/mL. Sensitivity: 0.19ng/mL

Intra-Assay CV: <=5.5%. Inter-Assay CV: <=8.9%. Recovery: 101%

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Components and Storage. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

7019499

NP_037393.1

NM_013261.3

Q9UBK2

77,139 Da

AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); ATF-2 Transcription Factor Network Pathway (138006); Activation Of PPARGC1A (PGC-1alpha) By Phosphorylation Pathway (1268840); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198832); BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway (1269872); Circadian Clock Pathway (1269871); Developmental Biology Pathway (1270302)

The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity. [provided by RefSeq, Jul 2008]

PGC-1 alpha: a transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor genes. Interacts with, and regulates the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). Regulates many genes involved in energy metabolism. Provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. May be involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity. Its transcription is repressed by ZNF746 which binds to 'insulin response sequences' in its promoter. Heavily acetylated by GCN5 and biologically inactive under conditions of high nutrients. Deacetylated by SIRT1 in low nutrients/high NAD conditions. Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor. Chromosomal Location of Human Ortholog: 4p15.1. Cellular Component: cell soma; DNA-directed RNA polymerase II, core complex; nucleoplasm; nucleus; PML body. Molecular Function: alpha-tubulin binding; androgen receptor binding; chromatin DNA binding; DNA binding; estrogen receptor binding; ligand-dependent nuclear receptor binding; ligand-dependent nuclear receptor transcription coactivator activity; nucleotide binding; peroxisome proliferator activated receptor binding; protein binding; RNA binding; sequence-specific DNA binding; transcription coactivator activity; transcription factor binding; ubiquitin protein ligase binding. Biological Process: aging; androgen metabolic process; androgen receptor signaling pathway; brown fat cell differentiation; cell glucose homeostasis; cellular respiration; cerebellum development; circadian regulation of gene expression; circadian rhythm; digestion; fatty acid oxidation; flavone metabolic process; forebrain development; galactose metabolic process; gluconeogenesis; mitochondrion degradation; mitochondrion organization and biogenesis; mRNA processing; negative regulation of glycolysis; negative regulation of neuron apoptosis; negative regulation of protein amino acid phosphorylation; negative regulation of smooth muscle cell migration; negative regulation of smooth muscle cell proliferation; organelle organization and biogenesis; positive regulation of fatty acid oxidation; positive regulation of gluconeogenesis; positive regulation of histone acetylation; positive regulation of smooth muscle cell proliferation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter by pheromones; positive regulation of transcription, DNA-dependent; protein complex assembly; protein stabilization; regulation of circadian rhythm; regulation of transcription, DNA-dependent; response to cold; response to dietary excess; response to electrical stimulus involved in regulation of muscle adaptation; response to leucine; response to muscle activity; response to reactive oxygen species; response to starvation; RNA splicing; skeletal muscle atrophy; thermoregulation; transcription initiation from RNA polymerase II promoter

48-Strip-Wells

355 USD

96-Strip-Wells

475

5x96-Strip-Wells

2030

10x96-Strip-Wells

3700