catalog number :
MBS2884409
products type :
ELISA Kit
products full name :
Mouse Eosinophil peroxidase ELISA Kit
products short name :
[Eosinophil peroxidase]
products name syn :
[Eosinophil peroxidase; EPO; Epx; Eper; 1.11.1.7]
other names :
[eosinophil peroxidase; Eosinophil peroxidase; eosinophil peroxidase; eosinophil peroxidase]
products gene name :
[Epx]
other gene names :
[Epx; Epx; EPO; Eper; EPO]
uniprot entry name :
PERE_MOUSE
specificity :
Recombinant and natural Mouse Fumarylacetoacetase,FAA
storage stability :
For long term storage, please store the entire kit at -20 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids. Detection Range: 0.156-10 ng/mL. Sensitivity: <0.048 ng/mL
other info2 :
Intra-assay Precision: Intra-Assay CV: <=7.1%. Inter-assay Precision: Inter-Assay CV: <=10.4%
products description :
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_031972.2
ncbi gb acc num :
NM_007946.2
ncbi mol weight :
81,380 Da
ncbi pathways :
Asthma Pathway (83313); Asthma Pathway (532); Phosphate Acquisition II Pathway (542835)
uniprot summary :
EPX: Mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils. Shows significant inhibitory activity towards Mycobacterium tuberculosis H37Rv by inducing bacterial fragmentation and lysis. Defects in EPX are the cause of eosinophil peroxidase deficiency (EPD). EPD is an autosomal recessive defect where anomalous eosinophils are characterized by nuclear hypersegmentation, hypogranulation, and negative peroxidase and phospholipid staining. Belongs to the peroxidase family. XPO subfamily. Protein type: EC 1.11.1.7; Oxidoreductase. Molecular Function: heme binding; metal ion binding; oxidoreductase activity; peroxidase activity. Biological Process: defense response; defense response to nematode; hydrogen peroxide catabolic process; negative regulation of interleukin-10 production; negative regulation of interleukin-5 production; positive regulation of interleukin-4 production; response to oxidative stress
size4 :
10x96-Strip-Wells