ELISA/assay

Mouse Insulin-degrading enzyme ELISA Kit

MBS2884110

48-Strip-Wells

355 USD

ELISA Kit

Mouse Insulin-degrading enzyme ELISA Kit

[Insulin-degrading enzyme]

[Insulin-degrading enzyme; Insulin protease; Insulinase; Insulysin; Ide; 3.4.24.56]

[Insulin-degrading enzyme; Insulin-degrading enzyme; insulin-degrading enzyme; insulin degrading enzyme; Insulin protease; Insulinase; Insulysin]

[Ide]

[Ide; Ide; AA675336; AI507533; 1300012G03Rik; 4833415K22Rik; Insulinase]

IDE_MOUSE

Mouse

1019

Recombinant and natural Human Netrin receptor UNC5C

For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids. Detection Range: 0.156-10 ng/mL. Sensitivity: <0.056 ng/mL

Intra-assay Precision: Intra-Assay CV: <=5.3%. Inter-assay Precision: Inter-Assay CV: <=7.9%

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

14548072

Q9JHR7.1

Q9JHR7

117,772 Da

Alzheimer's Disease Pathway (83294); Alzheimer's Disease Pathway (509)

IDE: Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia. Belongs to the peptidase M16 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.4.24.56; Cell surface; Nuclear receptor co-regulator; Protease. Cellular Component: cell surface; cytoplasm; cytosol; extracellular region; extracellular space; membrane; mitochondrion; nucleoplasm; nucleus; peroxisomal matrix; peroxisome; plasma membrane. Molecular Function: ATP binding; ATPase activity; beta-amyloid binding; beta-endorphin binding; catalytic activity; glycoprotein binding; hydrolase activity; insulin binding; metal ion binding; metalloendopeptidase activity; metallopeptidase activity; nucleotide binding; peptidase activity; peptide binding; peptide hormone binding; protein homodimerization activity; receptor binding; ubiquitin binding; zinc ion binding. Biological Process: beta-amyloid metabolic process; cellular protein catabolic process; determination of adult life span; hormone catabolic process; metabolic process; negative regulation of proteolysis; peptide catabolic process; positive regulation of protein oligomerization; protein heterooligomerization; protein homooligomerization; protein homotetramerization; protein processing; proteolysis; proteolysis involved in cellular protein catabolic process

48-Strip-Wells

355 USD

96-Strip-Wells

475

5x96-Strip-Wells

2030

10x96-Strip-Wells

3700