Mouse Autophagy protein 5 ELISA Kit | MyBioSource MBS2884012 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Autophagy protein 5 ELISA Kit

catalog :

MBS2884012

quantity :

48-Strip-Wells

price :

355 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2884012

products type :

ELISA Kit

products full name :

Mouse Autophagy protein 5 ELISA Kit

products short name :

[Autophagy protein 5]

products name syn :

[Autophagy protein 5; APG5-like; Atg5; Apg5l]

other names :

[autophagy protein 5 isoform 2; Autophagy protein 5; autophagy protein 5; autophagy related 5; APG5-like]

products gene name :

[Atg5]

other gene names :

[Atg5; Atg5; Apg5l; Atg5l; Paddy; C88337; AW319544; 2010107M05Rik; 3110067M24Rik; Apg5l]

uniprot entry name :

ATG5_MOUSE

reactivity :

Mouse

sequence length :

171

storage stability :

For long term storage, please store the entire kit at -20 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids.

products description :

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

930155595

ncbi acc num :

NP_001300942.1

ncbi gb acc num :

NM_001314013.1

uniprot acc num :

Q99J83

ncbi mol weight :

32,402 Da

ncbi pathways :

Cellular Responses To Stress Pathway (1324506); Longevity Regulating Pathway - Mammal (1320038); Longevity Regulating Pathway - Multiple Species (1340727); Longevity Regulating Pathway - Multiple Species (1340651); Macroautophagy Pathway (1324524); Mitophagy Pathway (1324498); Pink/Parkin Mediated Mitophagy Pathway (1324499); PodNet: Protein-protein Interactions In The Podocyte Pathway (755428); RIG-I-like Receptor Signaling Pathway (117302); RIG-I-like Receptor Signaling Pathway (116128)

ncbi summary :

The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Two transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

uniprot summary :

ATG5: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. The ATG5-ATG12 conjugate forms a complex with several units of ATG16. Interacts with TECPR1; the interaction is direct and does not take place when ATG16 is associated with the ATG5- ATG12 conjugate. By apoptotic stimuli. Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. Belongs to the ATG5 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Autophagy; Apoptosis; Ubiquitin conjugating system. Cellular Component: autophagic vacuole; axoneme; cytoplasm; membrane; pre-autophagosomal structure membrane. Molecular Function: APG8 conjugating enzyme activity; protein binding. Biological Process: antigen processing and presentation of endogenous antigen; apoptosis; autophagic vacuole formation; autophagy; blood vessel remodeling; cellular homeostasis; cellular response to nitrogen starvation; cellular response to starvation; heart contraction; immune system process; innate immune response; macroautophagy; mitochondrion degradation; negative regulation of apoptosis; negative regulation of phagocytosis; negative regulation of protein ubiquitination; negative thymic T cell selection; otolith development; post-translational protein modification; regulation of cytokine secretion during immune response; regulation of release of sequestered calcium ion into cytosol; response to drug; response to fungus; vasodilation; ventricular cardiac muscle cell development

size1 :

48-Strip-Wells

price1 :

355 USD

size2 :

96-Strip-Wells

price2 :

475

size3 :

5x96-Strip-Wells

price3 :

2030

size4 :

10x96-Strip-Wells

price4 :

3700

more info or order :

MyBioSource product webpage