ELISA/assay

Human High mobility group protein B1 ELISA Kit

MBS2883339

48-Strip-Wells

355 USD

ELISA Kit

Human High mobility group protein B1 ELISA Kit

[High mobility group protein B1]

[High mobility group protein B1; High mobility group protein 1; HMG-1; HMGB1; HMG1]

[high mobility group protein B1; High mobility group protein B1; High mobility group protein 1; HMG-1]

[HMGB1]

[HMGB1; HMG1; HMG-1]

HMGB1_HUMAN

Human

215

Recombinant and natural Human High mobility group protein B1 (HMGB1)

For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids.

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

926459555

NP_001300821.1

NM_001313892.1

P09429

24,894 Da

Activated TLR4 Signalling Pathway (1269236); Activation Of DNA Fragmentation Factor Pathway (1270293); Advanced Glycosylation Endproduct Receptor Signaling Pathway (1269258); Apoptosis Pathway (1270262); Apoptosis Induced DNA Fragmentation Pathway (1270292); Apoptotic Execution Phase Pathway (1270287); Base Excision Repair Pathway (83043); Base Excision Repair Pathway (451); Cytosolic Sensors Of Pathogen-associated DNA Pathway (1269268); DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway (1269276)

HMGB1: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Belongs to the HMGB family. Protein type: DNA repair, damage; Nuclear receptor co-regulator. Chromosomal Location of Human Ortholog: 13q12. Cellular Component: cell surface; condensed chromosome; cytosol; early endosome; ER-Golgi intermediate compartment; extracellular region; extracellular space; nucleoplasm; nucleus; plasma membrane; transcriptional repressor complex. Molecular Function: bubble DNA binding; C-X-C chemokine binding; chemoattractant activity; cytokine activity; damaged DNA binding; DNA bending activity; double-stranded DNA binding; double-stranded RNA binding; four-way junction DNA binding; lipopolysaccharide binding; lyase activity; phosphatidylserine binding; protein binding; RAGE receptor binding; single-stranded DNA binding; single-stranded RNA binding; transcription factor activity; transcription factor binding. Biological Process: activation of innate immune response; apoptosis; apoptotic cell clearance; autophagy; base-excision repair; cell structure disassembly during apoptosis; chromatin assembly; dendritic cell chemotaxis; DNA fragmentation during apoptosis; DNA geometric change; DNA ligation during DNA repair; DNA recombination; DNA topological change; elevation of cytosolic calcium ion concentration; endothelial cell proliferation; inflammatory response; inflammatory response to antigenic stimulus; innate immune response; macrophage activation during immune response; myeloid dendritic cell activation; negative regulation of blood vessel endothelial cell migration; negative regulation of CD4-positive, alpha beta T cell differentiation; negative regulation of interferon-gamma production; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcriptional preinitiation complex assembly; neurite development; plasmacytoid dendritic cell activation; positive chemotaxis; positive regulation of activated T cell proliferation; positive regulation of apoptosis; positive regulation of caspase activity; positive regulation of DNA binding; positive regulation of DNA ligation; positive regulation of interferon-alpha production; positive regulation of interferon-beta production; positive regulation of interleukin-1 beta secretion; positive regulation of interleukin-1 secretion; positive regulation of interleukin-10 production; positive regulation of interleukin-12 production; positive regulation of JNK cascade; positive regulation of MAPKKK cascade; positive regulation of mismatch repair; positive regulation of toll-like receptor 2 signaling pathway; positive regulation of toll-like receptor 4 signaling pathway; positive regulation of toll-like receptor 9 signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tumor necrosis factor production; programmed cell death; regulation of autophagy; regulation of restriction endodeoxyribonuclease activity; regulation of T cell mediated immune response to tumor cell; regulation of tolerance induction; regulation of transcription from RNA polymerase II promoter; T-helper 1 cell differentiation; V(D)J recombination

48-Strip-Wells

355 USD

96-Strip-Wells

475

5x96-Strip-Wells

2030

10x96-Strip-Wells

3700