Mouse Norrin ELISA Kit | MyBioSource MBS2883083 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Norrin ELISA Kit

catalog :

MBS2883083

quantity :

48-Strip-Wells

price :

400 USD

product information

catalog number :

MBS2883083

products type :

ELISA Kit

products full name :

Mouse Norrin ELISA Kit

products short name :

[Norrin]

products name syn :

[Norrin; Norrie disease protein homolog; Ndp; Ndph]

other names :

[norrin; Norrin; norrin; Norrie disease (pseudoglioma) (human); Norrie disease protein homolog]

products gene name :

[Ndp]

other gene names :

[Ndp; Ndp; ND; Ndph; Ndph]

uniprot entry name :

NDP_MOUSE

reactivity :

Mouse

sequence length :

131

specificity :

Recombinant and natural Mouse Norrin

storage stability :

For long term storage, please store the entire kit at -20 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Assay Type: Sandwich. Detection Range: 0.312-20 ng/mL. Sensitivity: 0.156 ng/mL

other info2 :

Intra-Assay CV: <=5.2%. Inter-Assay CV: <=8.1%. Recovery: 101%

products description :

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

6754808

ncbi acc num :

NP_035013.1

ncbi gb acc num :

NM_010883.3

uniprot acc num :

P48744

ncbi mol weight :

14,700 Da

ncbi summary :

This gene encodes a secreted protein that acts a ligand for multiple different receptors and participates in both Wnt and Wnt-independant signalling. Its activity is important in vascular development of the eye and inner ear. Mutation of the related gene in humans causes Norrie syndrome. [provided by RefSeq, May 2015]

uniprot summary :

NDP: Activates the canonical Wnt signaling pathway through FZD4 and LRP5 coreceptor. Plays a central role in retinal vascularization by acting as a ligand for FZD4 that signals via stabilizing beta-catenin (CTNNB1) and activating LEF/TCF-mediated transcriptional programs. Acts in concert with TSPAN12 to activate FZD4 independently of the Wnt-dependent activation of FZD4, suggesting the existence of a Wnt-independent signaling that also promote accumulation the beta-catenin (CTNNB1). May be involved in a pathway that regulates neural cell differentiation and proliferation. Possible role in neuroectodermal cell-cell interaction. Defects in NDP are the cause of Norrie disease (ND); also known as atrophia bulborum hereditaria or Episkopi blindness. ND is a recessive disorder characterized by very early childhood blindness due to degenerative and proliferative changes of the neuroretina. Approximately 50% of patients show some form of progressive mental disorder, often with psychotic features, and about one-third of patients develop sensorineural deafness in the second decade. In addition, some patients have more complex phenotypes, including growth failure and seizure. Defects in NDP are the cause of vitreoretinopathy exudative type 2 (EVR2). EVR2 is a disorder of the retinal vasculature characterized by an abrupt cessation of growth of peripheral capillaries, leading to an avascular peripheral retina. This may lead to compensatory retinal neovascularization, which is thought to be induced by hypoxia from the initial avascular insult. New vessels are prone to leakage and rupture causing exudates and bleeding, followed by scarring, retinal detachment and blindness. Clinical features can be highly variable, even within the same family. Patients with mild forms of the disease are asymptomatic, and their only disease related abnormality is an arc of avascular retina in the extreme temporal periphery. Protein type: Secreted, signal peptide; Secreted. Cellular Component: cell surface; extracellular matrix; extracellular region; extracellular space. Molecular Function: cytokine activity; frizzled binding; protein homodimerization activity. Biological Process: placenta development; positive regulation of transcription factor activity; positive regulation of transcription, DNA-dependent; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin

size1 :

48-Strip-Wells

price1 :

400 USD

size2 :

96-Strip-Wells

price2 :

530

size3 :

5x96-Strip-Wells

price3 :

2305

size4 :

10x96-Strip-Wells

price4 :

4250