2,3-Bisphosphoglycerate ELISA Kit | MyBioSource MBS288269 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

2,3-Bisphosphoglycerate ELISA Kit

catalog :

MBS288269

quantity :

48-Strip-Wells

price :

400 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS288269

products type :

ELISA Kit

products full name :

2,3-Bisphosphoglycerate ELISA Kit

products short name :

[2,3-Bisphosphoglycerate]

other names :

[2,3-bisphosphoglycerate; Bisphosphoglycerate mutase; bisphosphoglycerate mutase; bisphosphoglycerate mutase; 2,3-bisphosphoglycerate mutase, erythrocyte; 2,3-bisphosphoglycerate synthase (EC:3.1.3.13, EC:5.4.2.11); 2,3-diphosphoglycerate mutase; DPGM; BPG-dependent PGAM]

products gene name :

[2,3-BPG]

other gene names :

[BPGM; BPGM; DPGM; BPGM; DPGM]

uniprot entry name :

PMGE_HUMAN

reactivity :

General

sequence length :

259

specificity :

Recombinant and natural General Endotoxin Synonyms: Endotoxin

storage stability :

For long term storage, please store the entire kit at -20 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 0.015-1.0 EU/mL. Sensitivity: <0.005 EU/mL

other info2 :

Intra-assay Precision: Intra-Assay CV: <=4.3%. Inter-assay Precision: Inter-Assay CV: <=5.5%

products description :

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The ELISA is based on the competitive binding enzyme immuncassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen, During the reaction, target antigen in the sample or standard competes with a fixed amount of biotin-labeled target antigen for sites on a pre-coated antibody specific to target antigen. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectro-photometrically at a wavelength of 450 nm +/- 2nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

179527

ncbi acc num :

AAA51840.1

ncbi mol weight :

30,005 Da

ncbi pathways :

Glycine, Serine And Threonine Metabolism Pathway (82949); Glycine, Serine And Threonine Metabolism Pathway (313); Glycolysis / Gluconeogenesis Pathway (82926); Glycolysis / Gluconeogenesis Pathway (287); Metabolic Pathways (132956); Rapoport-Luebering Glycolytic Shunt Pathway (545320); Rapoport-Luebering Glycolytic Shunt Pathway (545483); Gluconeogenesis Pathway (782382); Gluconeogenesis III Pathway (908097); Glycolysis Pathway (782396)

ncbi summary :

2,3-diphosphoglycerate (2,3-DPG) is a small molecule found at high concentrations in red blood cells where it binds to and decreases the oxygen affinity of hemoglobin. This gene encodes a multifunctional enzyme that catalyzes 2,3-DPG synthesis via its synthetase activity, and 2,3-DPG degradation via its phosphatase activity. The enzyme also has phosphoglycerate phosphomutase activity. Deficiency of this enzyme increases the affinity of cells for oxygen. Mutations in this gene result in hemolytic anemia. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Sep 2009]

uniprot summary :

BPGM: Plays a major role in regulating hemoglobin oxygen affinity by controlling the levels of its allosteric effector 2,3- bisphosphoglycerate (2,3-BPG). Also exhibits mutase (EC 5.4.2.1) and phosphatase (EC 3.1.3.13) activities. Defects in BPGM are the cause of bisphosphoglycerate mutase deficiency (BPGMD). A disease characterized by hemolytic anemia, splenomegaly, cholelithiasis and cholecystitis. Belongs to the phosphoglycerate mutase family. BPG- dependent PGAM subfamily. Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 5.4.2.4; EC 3.1.3.13; Phosphatase (non-protein); Isomerase; EC 5.4.2.11. Chromosomal Location of Human Ortholog: 7q33. Cellular Component: cytosol. Molecular Function: 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase activity; bisphosphoglycerate mutase activity; bisphosphoglycerate phosphatase activity. Biological Process: carbohydrate metabolic process; dephosphorylation; gluconeogenesis; glycolysis; regulation of pentose-phosphate shunt; respiratory gaseous exchange. Disease: Bisphosphoglycerate Mutase Deficiency

size1 :

48-Strip-Wells

price1 :

400 USD

size2 :

96-Strip-Wells

price2 :

530

size3 :

5x96-Strip-Wells

price3 :

2305

size4 :

10x96-Strip-Wells

price4 :

4250

more info or order :

MyBioSource product webpage