ELISA/assay

Testosterone ELISA Kit

MBS288265

48-Strip-Wells

245 USD

ELISA Kit

Testosterone ELISA Kit

[Testosterone]

General

This assay recognizes recombinant and natural General Testosterone. No significant cross-reactivity or interference was observed.

For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids. Assay Type: Quantitative Competitive

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The ELISA is based on the competitive binding enzyme immuncassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen, During the reaction, target antigen in the sample or standard competes with a fixed amount of biotin-labeled target antigen for sites on a pre-coated antibody specific to target antigen. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectro-photometrically at a wavelength of 450 nm +/- 2nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

48-Strip-Wells

245 USD

96-Strip-Wells

300

5x96-Strip-Wells

1140

10x96-Strip-Wells

1930