ELISA/assay

Mouse Cathepsin K ELISA Kit

MBS2882088

48-Strip-Wells

355 USD

ELISA Kit

Mouse Cathepsin K ELISA Kit

[Cathepsin K]

[Cathepsin K; Ctsk; 3.4.22.38]

[cathepsin K preproprotein; Cathepsin K; cathepsin K; cathepsin K]

[Ctsk]

[Ctsk; Ctsk; catK; Ms10q; MMS10-Q; AI323530]

CATK_MOUSE

Mouse

329

For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Assay Type: Sandwich. Detection Range: 0.156-10 ng/mL. Sensitivity: 0.083 ng/mL

Intra-Assay CV: <=5.9%. Inter-Assay CV: <=9.1%. Recovery: 104%

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

31982433

NP_031828.2

NM_007802.4

P55097

36,889 Da

Activation Of Matrix Metalloproteinases Pathway (1323923); Collagen Degradation Pathway (1323924); Degradation Of The Extracellular Matrix Pathway (1323922); Extracellular Matrix Organization Pathway (1323909); Immune System Pathway (1323639); Innate Immune System Pathway (1323671); Lysosome Pathway (99272); Lysosome Pathway (96865); Osteoclast Pathway (198284); Osteoclast Differentiation Pathway (193149)

This gene encodes a member of the cathepsin family of cysteine proteases that is highly expressed in osteoclasts and is involved in the degradation of collagen and other matrix proteins in bone. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional enzyme. Mice lacking the encoded protein exhibit phenotypic features of pycnodysostosis such as increased bone density and bone deformity, which become progressively more pronounced with age. [provided by RefSeq, Jan 2016]

CTSK: Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. Defects in CTSK are the cause of pycnodysostosis (PKND). PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature. Belongs to the peptidase C1 family. Protein type: Protease; EC 3.4.22.38. Cellular Component: cytoplasm; extracellular space; lysosome. Molecular Function: collagen binding; cysteine-type endopeptidase activity; cysteine-type peptidase activity; fibronectin binding; hydrolase activity; peptidase activity; proteoglycan binding. Biological Process: bone resorption; collagen catabolic process; proteolysis; proteolysis involved in cellular protein catabolic process

48-Strip-Wells

355 USD

96-Strip-Wells

475

5x96-Strip-Wells

2030

10x96-Strip-Wells

3700