catalog number :
MBS2881978
products type :
ELISA Kit
products full name :
Pig Cholecystokinin ELISA Kit
products short name :
[Cholecystokinin]
products name syn :
[Cholecystokinin; CCK; CCK]
other names :
[cholecystokinin; Cholecystokinin; cholecystokinin; Cholecystokinin-58; CCK58]
products gene name :
[CCK]
other gene names :
[CCK; CCK; CCK; CCK58; CCK39; CCK33; CCK25; CCK18; CCK12; CCK8; CCK7; CCK5]
uniprot entry name :
CCKN_PIG
storage stability :
For long term storage, please store the entire kit at -20 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Assay Type: Competitive
products description :
Intended Uses This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen, During the reaction, target antigen in the sample or standard competes with a fixed amount of biotin-labeled target antigen for sites on a pre-coated antibody specific to target antigen. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_999402.1
ncbi gb acc num :
NM_214237.2
ncbi mol weight :
12,526 Da
ncbi pathways :
Class A/1 (Rhodopsin-like Receptors) Pathway (1337475); G Alpha (q) Signalling Events Pathway (1337507); GPCR Downstream Signaling Pathway (1337503); GPCR Ligand Binding Pathway (1337474); Gastrin-CREB Signalling Pathway Via PKC And MAPK (1337519); Peptide Ligand-binding Receptors Pathway (1337476); Signal Transduction Pathway (1337326); Signaling By GPCR Pathway (1337473)
uniprot summary :
This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion.
size4 :
10x96-Strip-Wells