catalog number :
MBS2881830
products type :
ELISA Kit
products full name :
Rat Calpastatin ELISA Kit
products short name :
[Calpastatin]
products name syn :
[Calpastatin; Calpain inhibitor; Cast]
other names :
[calpastatin isoform b; Calpastatin; calpastatin; calpastatin; Calpain inhibitor]
products gene name :
[Cast]
other gene names :
[Cast; Cast]
uniprot entry name :
ICAL_RAT
storage stability :
For long term storage, please store the entire kit at -20 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
products description :
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_001028887.1
ncbi gb acc num :
NM_001033715.1
ncbi mol weight :
58,859 Da
ncbi summary :
inhibitor of calpain; degradation is required for myoblast fusion; exists in multiple alternatively spliced isoforms [RGD, Feb 2006]
uniprot summary :
calpastatin: Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue. Belongs to the protease inhibitor I27 (calpastatin) family. 7 isoforms of the human protein are produced by alternative splicing. Protein type: Inhibitor. Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; membrane; nucleus. Molecular Function: protease binding; protein binding. Biological Process: aging; brain development; egg activation; liver development; myoblast differentiation; myoblast fusion; negative regulation of catalytic activity; negative regulation of peptidase activity; organ regeneration; regulation of protein catabolic process
size4 :
10x96-Strip-Wells