ELISA/assay

Human Neurosecretory protein VGF ELISA Kit

MBS2881506

48-Strip-Wells

355 USD

ELISA Kit

Human Neurosecretory protein VGF ELISA Kit

[Neurosecretory protein VGF]

[Neurosecretory protein VGF; VGF]

[neurosecretory protein VGF; Neurosecretory protein VGF; neurosecretory protein VGF; VGF nerve growth factor inducible]

[VGF]

[VGF; VGF; SCG7; SgVII; NERP-1; NERP-2]

VGF_HUMAN

Human

615

For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Assay Type: Sandwich. Detection Range: 78-5000 pg/mL. Sensitivity: 32pg/mL

Intra-Assay CV: <=3.2%. Inter-Assay CV: <=6.8%. Recovery: 97%

Intended Uses This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

17136078

NP_003369.2

NM_003378.3

O15240

67,258 Da

This gene is specifically expressed in a subpopulation of neuroendocrine cells, and is upregulated by nerve growth factor. The structural organization of this gene is similar to that of the rat gene, and both the translated and the untranslated regions show a high degree of sequence similarity to the rat gene. The encoded secretory protein also shares similarities with the secretogranin/chromogranin family, however, its exact function is not known. [provided by RefSeq, Jul 2008]

VGF: May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 7q22.1. Cellular Component: cytoplasmic vesicle; extracellular space; intracellular membrane-bound organelle; transport vesicle. Molecular Function: growth factor activity; hormone activity; neuropeptide hormone activity. Biological Process: carbohydrate homeostasis; defense response to bacterium; generation of precursor metabolites and energy; glucose homeostasis; insulin secretion; ovarian follicle development; regulation of synaptic plasticity; response to cAMP; response to cold; response to dietary excess; response to insulin stimulus; sexual reproduction

48-Strip-Wells

355 USD

96-Strip-Wells

475

5x96-Strip-Wells

2030

10x96-Strip-Wells

3700